Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions.
Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions.
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DOI：
10.1016/j.biomaterials.2010.04.060
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发表时间：
2010-09
期刊：
影响因子：
14
通讯作者：
Tay FR
中科院分区：
文献类型：
--
作者：
Kim YK;Gu LS;Bryan TE;Kim JR;Chen L;Liu Y;Yoon JC;Breschi L;Pashley DH;Tay FR
矿化胶原原纤维的复杂形态是通过胶原基质和非胶原细胞外蛋白之间的相互作用来调节的。本研究合成了一种基质磷蛋白的仿生类似物——聚乙烯膦酸，并用FTIR和NMR对其进行了确证。利用含波特兰水泥的树脂复合材料和含磷酸盐的液体，在聚丙烯酸作为隔离剂和聚乙烯膦酸作为模板基质蛋白质类似物的情况下，用透射电镜证明了重建胶原原纤维的仿生矿化，而不含天然非胶原蛋白。在矿化介质中存在这些双仿生类似物时，可以通过基于非经典结晶途径的自下而上纳米颗粒组装来识别纤维内和纤维外的矿化。相反，在培养基中没有仿生类似物的情况下，只观察到与胶原原纤维没有首选关联的大矿物球。在初始形成的无定形磷酸钙纳米递质转化为磷灰石纳米晶体后4小时，胶原原纤维中就出现了明显的矿物相。高度矿化的胶原原纤维的选定区域电子衍射图与天然骨几乎相同，磷灰石晶体优先沿胶原原纤维轴排列。
The complex morphologies of mineralised collagen fibrils are regulated through interactions between the collagen matrix and non-collagenous extracellular proteins. In the present study, polyvinylphosphonic acid, a biomimetic analogue of matrix phosphoproteins, was synthesised and confirmed with FTIR and NMR. Biomimetic mineralisation of reconstituted collagen fibrils devoid of natural non-collagenous proteins was demonstrated with TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of polyacrylic acid as sequestration, and polyvinylphosphonic acid as templating matrix protein analogues. In the presence of these dual biomimetic analogues in the mineralisation medium, intrafibrillar and extrafibrillar mineralisation via bottom-up nanoparticle assembly based on the nonclassical crystallisation pathway could be identified. Conversely, only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium. Mineral phases were evident within the collagen fibrils as early as 4 hours after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals. Selected area electron diffraction patterns of highly mineralised collagen fibrils were nearly identical to those of natural bone, with apatite crystallites preferentially aligned along the collagen fibril axes.
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