Airway ciliated cells regenerated on collagen sponge implants acquire planar polarities towards nearby edges of implanted areas
Airway ciliated cells regenerated on collagen sponge implants acquire planar polarities towards nearby edges of implanted areas
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胶原海绵植入物上再生的气道纤毛细胞获得朝向植入区域附近边缘的平面极性
DOI：
10.1002/term.3220
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发表时间：
2021
影响因子：
3.3
通讯作者：
Omori Koichi
中科院分区：
文献类型：
--
作者：
Nakamura Ryosuke;Katsuno Tatsuya;Tsuji Takuya;Oyagi Seiji;Kishimoto Yo;Suehiro Atsushi;Tateya Ichiro;Omori Koichi
组织工程化气管已被开发用于替代有缺陷的气管。然而，再生上皮中纤毛细胞的方向仍不清楚。我们研究了气管移植物植入后再生气道上皮中形成的平面极性。我们切除了部分大鼠气管并植入了胶原支架。基底足的方向进行了评估，通过透射电子显微镜。进行免疫荧光染色以检查Vangl 1和Frizzled6蛋白的偏向性分布。通过视频显微镜分析粘液纤毛运输的方向。结果表明，植入区近端和远端纤毛基底足分别朝向近端和远端。Vangl1和Frizzled6的偏向性分布以及粘液纤毛运输的方向表明，在植入区域的近端、远端、左侧和右侧区域中，再生上皮中形成的平面极性朝向近端、远端、左侧和右侧方向。这些极性一直持续到植入后9个月。因此，结果表明，在气管移植物上再生的上皮中形成的平面极性指向植入区域的附近边缘，并被保存较长时间。极性可以至少部分地有助于通过将外部材料输送到正常区域来从注入区域清除外部材料。
Tissue‐engineered tracheae have been developed to replace defective tracheae. However, the direction of ciliated cells in the regenerated epithelium remains unclear. We investigated planar polarity formed in the regenerated airway epithelium after tracheal graft implantation. We partially resected the rat trachea and implanted a collagen scaffold. The direction of the basal foot was assessed by transmission electron microscopy. Immunofluorescence staining was performed to examine the biased distribution of Vangl1 and Frizzled6 proteins. The direction of mucociliary transport was analyzed by video microscopy. Our results showed that the basal feet of cilia in the proximal and distal regions of the implanted areas were respectively oriented toward the proximal and distal directions. The biased distribution of Vangl1 and Frizzled6, and the directions of mucociliary transport showed that planar polarities formed in the regenerated epithelium were oriented toward the proximal, distal, left, and right directions in the proximal, distal, left, and right regions of the implanted area. These polarities persisted until nine months after implantation. Hence, the results suggest that planar polarities formed in epithelia regenerated on tracheal grafts are directed toward the nearby edges of implanted areas and are preserved for a prolonged period. The polarities can, at least partially, contribute to clearing external materials from the implanted areas by transporting them to a normal region.
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DOI：
10.1146/annurev-cellbio-100814-125315
发表时间：
2015
影响因子：
11.3
作者：
Yang Y;Mlodzik M
通讯作者：
Mlodzik M
影响因子：
3.9
作者：
Vladar EK;Königshoff M
通讯作者：
Königshoff M
影响因子：
19
作者：
Vladar, Eszter K.;Axelrod, Jeffrey D.
通讯作者：
Axelrod, Jeffrey D.
DOI：
--
发表时间：
1956
期刊：
Surgery, gynecology & obstetrics
影响因子：
--
作者：
N. Correll;E. Beattie
通讯作者：
E. Beattie
影响因子：
2.6
作者：
DeLaere, PR;Liu, ZY;Feenstra, L
通讯作者：
Feenstra, L