In vivo speciation studies and antioxidant properties of bromine in Laminaria digitata reinforce the significance of iodine accumulation for kelps.
In vivo speciation studies and antioxidant properties of bromine in Laminaria digitata reinforce the significance of iodine accumulation for kelps.
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DOI：
10.1093/jxb/ert110
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发表时间：
2013-07
影响因子：
6.9
通讯作者：
Feiters MC
中科院分区：
文献类型：
--
作者：
Küpper FC;Carpenter LJ;Leblanc C;Toyama C;Uchida Y;Maskrey BH;Robinson J;Verhaeghe EF;Malin G;Luther GW 3rd;Kroneck PM;Kloareg B;Meyer-Klaucke W;Muramatsu Y;Megson IL;Potin P;Feiters MC
溴在海洋褐藻中的代谢仍然知之甚少。这与最近的发现形成鲜明对比，即棕色海带中碘的积累提供了无机抗氧化剂-这是第一个从生命系统中记录的案例。本研究的目的是使用一个跨学科阵列的技术来研究的化学形态，转化和功能的溴在海带和溴和碘代谢之间的联系，特别是在抗氧化剂的背景下进行调查。首先，在不同的海带组织中的溴和碘的水平进行了比较，电感耦合等离子体MS。使用体内X-射线吸收光谱法，它被发现，类似于碘，溴主要存在于此海带中的形式溴化物，虽然在较低的浓度，它显示出类似的行为后，氧化应激。然而，从热力学和动力学的角度来看，支持在体外和重建的体内测定，溴化物是不太适合比碘化物作为抗氧化剂对大多数活性氧物种，但超氧化物，可能解释了为什么海带喜欢积累碘化物。这是有史以来第一次探索溴化物在生命系统中的潜在抗氧化功能和其他潜在生理作用的研究。鉴于溴的含量和形态观察到的组织特异性差异，可以得出结论，溴吸收机制是不同的钒碘过氧化物酶介导的碘吸收在L。毛地黄，其功能可能是补充碘化物抗氧化系统解毒超氧化物。
The metabolism of bromine in marine brown algae remains poorly understood. This contrasts with the recent finding that the accumulation of iodide in the brown alga Laminaria serves the provision of an inorganic antioxidant – the first case documented from a living system. The aim of this study was to use an interdisciplinary array of techniques to study the chemical speciation, transformation, and function of bromine in Laminaria and to investigate the link between bromine and iodine metabolism, in particular in the antioxidant context. First, bromine and iodine levels in different Laminaria tissues were compared by inductively coupled plasma MS. Using in vivo X-ray absorption spectroscopy, it was found that, similarly to iodine, bromine is predominantly present in this alga in the form of bromide, albeit at lower concentrations, and that it shows similar behaviour upon oxidative stress. However, from a thermodynamic and kinetic standpoint, supported by in vitro and reconstituted in vivo assays, bromide is less suitable than iodide as an antioxidant against most reactive oxygen species except superoxide, possibly explaining why kelps prefer to accumulate iodide. This constitutes the first-ever study exploring the potential antioxidant function of bromide in a living system and other potential physiological roles. Given the tissue-specific differences observed in the content and speciation of bromine, it is concluded that the bromide uptake mechanism is different from the vanadium iodoperoxidase-mediated uptake of iodide in L. digitata and that its function is likely to be complementary to the iodide antioxidant system for detoxifying superoxide.
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影响因子：
2.5
作者：
Feiters, MC;Küpper, FC;Meyer-Klaucke, W
通讯作者：
Meyer-Klaucke, W
影响因子：
4.4
作者：
Carpenter, LJ;Liss, PS
通讯作者：
Liss, PS
影响因子：
3.9
作者：
KANOFSKY, JR;SIMA, PD
通讯作者：
SIMA, PD
影响因子：
4.9
作者：
Kuepper, Frithjof C.;Gaquerel, Emmanuel;Potin, Philippe
通讯作者：
Potin, Philippe
影响因子：
3.9
作者：
BHATTACHARYA, D;STICKEL, SK;SOGIN, ML
通讯作者：
SOGIN, ML