Synthesis and antibacterial activity of polymer-antibiotic conjugates incorporated into a resin-based dental adhesive.
Synthesis and antibacterial activity of polymer-antibiotic conjugates incorporated into a resin-based dental adhesive.
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DOI：
10.1039/d0bm01910k
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发表时间：
2021-03-21
影响因子：
6.6
通讯作者：
Cheng C
中科院分区：
文献类型：
--
作者：
Zhang Z ;Jones MM ;Sabatini C ;Vanyo ST ;Yang M ;Kumar A ;Jiang Y ;Swihart MT ;Visser MB ;Cheng C
这项工作报告了聚合物-抗生素结合物（PAC）作为树脂基修复牙科材料的添加剂，作为向这些材料传递持续抗菌特性的新策略。这种抗菌性能有望延长它们在口腔中的寿命。使用先前报道的基于环丙沙星 (Cip) 的 PAC 作为对照，研究了基于青霉素 V (PV) 的 PAC。通过甲基丙烯酸 2-氯乙酯 (CEMA) 和青霉素 V 钾 (PVK) 之间的亲核取代制备含有甲基丙烯酸酯单体基团和 PV 部分的单体-抗生素缀合物 (MAC)。通过 MAC 与甲基丙烯酸羟乙酯 (HEMA) 的可逆加成断裂链转移 (RAFT) 聚合合成了基于 PV 的 PAC，并通过 1H NMR 和凝胶渗透色谱 (GPC) 分析进一步表征。通过在低浓度抗生素下传代细菌 19 天，然后在较高浓度下进行 48 小时攻击来研究抗生素耐药性。我们的结果表明不太可能出现抗生素耐药性。抑制区 (ZOI) 测定显示含 PV 的树脂周围没有清除区，表明材料中的抗生素泄漏极少。同样，MTT 测定表明，含抗生素的标本不会释放可能抑制人牙龈成纤维细胞生长的细胞毒性副产物。对变形链球菌生物膜模型中的菌落形成单位进行计数，用于评估基线时和含抗生素树脂样本接受酶促攻击 30 天后的细菌存活率。随着生物膜老化 24 至 72 小时，观察到细菌数量显着减少，并且唾液酶暴露并没有降低椎间盘的抗菌功效，这表明 PV 树脂将有效减少龋齿的复发。与青霉素 V (PV) 基聚合物-抗生素结合物 (PAC) 结合的牙科树脂表现出显着的抗菌特性。
This work reports on polymer-antibiotic conjugates (PACs) as additives to resin-based restorative dental materials as a new strategy to convey sustained antibacterial character to these materials. Such antibacterial performance is expected to improve their longevity in the oral cavity. Using the previously reported ciprofloxacin (Cip)-based PAC as a control, a penicillin V (PV)-based PAC was investigated. The monomer-antibiotic conjugate (MAC) containing a methacrylate monomer group and a PV moiety was prepared via nucleophilic substitution between 2-chloroethyl methacrylate (CEMA) and penicillin V potassium (PVK). The PV-based PAC was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of the MAC with hydroxyethyl methacrylate (HEMA), and further characterized by 1H NMR and gel permeation chromatography (GPC) analysis. Antibiotic resistance was investigated by passaging bacteria in low concentrations of the antibiotic for 19 days, followed by a 48-h challenge at higher concentrations. Our results suggest that the development of antibiotic resistance is unlikely. Zone of inhibition (ZOI) assays revealed no clearing zones around PV-containing resins indicating minimal antibiotic leakage from the material. Similarly, MTT assay demonstrated that the antibiotic-containing specimens did not release cytotoxic byproducts that may inhibit human gingival fibroblast growth. Counting of colony-forming units in an S. mutans biofilm model was used to assess bacterial survival at baseline and after subjecting the antibiotic-containing resin specimens to an enzymatic challenge for 30 days. Significantly reduced bacterial counts were observed as the biofilm aged from 24 to 72 h, and salivary enzymatic exposure did not reduce the antibacterial efficacy of the discs, suggesting that PV-resin will be effective in reducing the re-incidence of dental caries. The dental resins incorporated with a penicillin V (PV)-based polymer-antibiotic conjugate (PAC) demonstrate significant antibacterial properties.
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DOI：
10.2147/ccide.s42044
发表时间：
2013
期刊：
Clinical, cosmetic and investigational dentistry
影响因子：
--
作者：
Bohaty BS;Ye Q;Misra A;Sene F;Spencer P
通讯作者：
Spencer P
影响因子：
4.9
作者：
BAKER, CN;THORNSBERRY, C
通讯作者：
THORNSBERRY, C
影响因子：
1.1
作者：
Fuentes-Paniagua, Elena;Hernandez-Ros, Jose M.;Javier de la Mata, F.
通讯作者：
Javier de la Mata, F.
影响因子：
5
作者：
Delaviz, Yasaman;Nascimento, Mitchell A.;Santerre, J. Paul
通讯作者：
Santerre, J. Paul
影响因子：
5
作者：
Beyth, Nurit;Bahir, Ran;Weiss, Ervin I.
通讯作者：
Weiss, Ervin I.