Biological Impact of Silicon Nitride for Orthopaedic Applications: Role of Particle Size, Surface Composition and Donor Variation.
Biological Impact of Silicon Nitride for Orthopaedic Applications: Role of Particle Size, Surface Composition and Donor Variation.
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DOI：
10.1038/s41598-018-27494-y
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发表时间：
2018-06-14
影响因子：
4.6
通讯作者：
Tipper JL
中科院分区：
文献类型：
--
作者：
Lal S;Caseley EA;Hall RM;Tipper JL
骨科磨损碎屑的不良生物学影响目前限制了人类关节置换器械的长期安全性。我们研究了粒度、表面组成和供体变化在影响氮化硅作为骨科应用生物陶瓷的生物学影响方面的作用。将氮化硅颗粒与其他常用骨科生物材料（例如钴铬合金和Ti-6Al-4V合金）进行比较。开发了一种新型生物学评价平台，使用来自个体人类供体的外周血单核细胞（PBMNC）同时评价颗粒的细胞毒性、炎性细胞因子释放、氧化应激和遗传毒性潜力。无论颗粒大小如何，氮化硅均未引起任何不良反应，而钴铬磨损颗粒在24 h后引起供体依赖性细胞毒性、TNF-α细胞因子释放、氧化应激和PBMC中的DNA损伤。尽管尺寸和形态相似，但与氮化硅纳米颗粒相比，二氧化硅纳米颗粒引起显著更高水平的TNF-α释放，表明表面组成影响PBMNCs中的炎症反应。Ti-6Al-4V磨损颗粒还在一个供体中释放了显著升高水平的TNF-α细胞因子。这项研究表明，氮化硅是一种有吸引力的骨科生物材料，因为它对人PBMNCs的生物学影响最小。
The adverse biological impact of orthopaedic wear debris currently limits the long-term safety of human joint replacement devices. We investigated the role of particle size, surface composition and donor variation in influencing the biological impact of silicon nitride as a bioceramic for orthopaedic applications. Silicon nitride particles were compared to the other commonly used orthopaedic biomaterials (e.g. cobalt-chromium and Ti-6Al-4V alloys). A novel biological evaluation platform was developed to simultaneously evaluate cytotoxicity, inflammatory cytokine release, oxidative stress, and genotoxicity potential of particles using peripheral blood mononuclear cells (PBMNCs) from individual human donors. Irrespective of the particle size, silicon nitride did not cause any adverse responses whereas cobalt-chromium wear particles caused donor-dependent cytotoxicity, TNF-α cytokine release, oxidative stress, and DNA damage in PBMNCs after 24 h. Despite being similar in size and morphology, silicon dioxide nanoparticles caused the release of significantly higher levels of TNF-α compared to silicon nitride nanoparticles, suggesting that surface composition influences the inflammatory response in PBMNCs. Ti-6Al-4V wear particles also released significantly elevated levels of TNF-α cytokine in one of the donors. This study demonstrated that silicon nitride is an attractive orthopaedic biomaterial due to its minimal biological impact on human PBMNCs.
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DOI：
10.1002/jbm.b.32904
发表时间：
2013-04
影响因子：
3.4
作者：
Bladen, C. L.;Teramura, S.;Russell, S. L.;Fujiwara, K.;Fisher, J.;Ingham, E.;Tomita, N.;Tipper, J. L.
通讯作者：
Tipper, J. L.
影响因子：
9.7
作者：
Liu A;Richards L;Bladen CL;Ingham E;Fisher J;Tipper JL
通讯作者：
Tipper JL
影响因子：
3.2
作者：
Jahanmir, S;Ozmen, Y;Ives, LK
通讯作者：
Ives, LK
影响因子：
4.9
作者：
Lewis, A. C.;Ladon, D.;Learmonth, I.
通讯作者：
Learmonth, I.
影响因子：
3.5
作者：
Bal, B. Sonny;Khandkar, Ashok;Rahaman, Mohamed N.
通讯作者：
Rahaman, Mohamed N.