Ethanol inhibits electrically-induced calcium transients in isolated rat cardiac myocytes.
Ethanol inhibits electrically-induced calcium transients in isolated rat cardiac myocytes.
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乙醇抑制离体大鼠心肌细胞中电诱导的钙瞬变。
DOI：
10.1016/0022-2828(89)90821-3
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发表时间：
1989
影响因子：
5
通讯作者：
Rubin,E
中科院分区：
文献类型：
--
作者：
Thomas,AP;Sass,EJ;Tun-Kirchmann,TT;Rubin,E
用钙离子荧光指示剂Fura-2跟踪电场刺激诱导的大鼠心肌细胞悬液在兴奋-收缩偶联过程中的胞浆钙瞬变。钙瞬变在施加电刺激后约30ms达到最大值，然后在接下来的200ms内松弛到基础水平。用0.25%~2.0%乙醇(40~340 mM)处理心肌细胞后，钙瞬变峰值降低，峰值时间无明显变化。在达到新的稳定状态之前，乙醇的这种效应在大约1分钟的时间内逐渐发生。在乙醇浓度为1%时，钙离子峰值降低了50%。乙醇可逆转异丙肾上腺素对钙离子峰值的刺激作用，在高浓度乙醇作用下，β肾上腺素能激动剂不再引起钙瞬变的增强。乙醇对心跳间期的基础钙水平无明显影响。乙醇的作用很容易逆转。这些结果表明，乙醇在完整心肌标本中观察到的负性变力作用可能部分是由于干扰了负责兴奋-收缩偶联的心肌细胞的钙离子通量。
The fluorescent Ca2+indicator fura-2 was used to follow cytosolic Ca2+transients during excitation-contraction coupling in suspensions of isolated rat heart cells induced to beat synchronously by electrical field stimulation. The Ca2+transient reached a maximum at about 30 ms after application of the electrical stimulus and then relaxed to the basal level over the following 200 ms. Treatment of the myocytes with 0.25 to 2.0% ethanol (40 to 340 mm) caused a decrease in the peak of the Ca2+transient, with no apparent change in the time to peak. This effect of ethanol occurred progressively over a period of about 1 min before a new stable state was achieved. At 1% ethanol the peak Ca2+level was reduced by 50%. Ethanol reversed the stimulatory effect of isoproterenol on peak Ca2+and at high levels of ethanol the β-adrenergic agonist no longer caused any enhancement of the Ca2+transient. Ethanol did not cause any marked change in the basal Ca2+level between beats. The effects of ethanol were readily reversible. These results suggest that the negative inotropic effect of ethanol observed in intact cardiac muscle preparations may result in part from interference with the Ca2+fluxes responsible for excitation-contraction coupling in ventricular myocytes.
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二磷酸腺苷对人肌肉肌动球蛋白收缩性的影响：乙醇和乙醛的抑制。
影响因子：
56.9
作者：
S. Puszkin;E. Rubin
通讯作者：
E. Rubin
酒精引起的心脏血流动力学和 Ca2+ 通量功能障碍是可逆的。
影响因子：
5
作者：
L. D. Segel;S. Rendig;D. Mason
通讯作者：
D. Mason
DOI：
10.1159/000469242
发表时间：
1987
期刊：
Enzyme
影响因子：
--
作者：
B. Tabakoff;P. Hoffman;S. Liljequist
通讯作者：
S. Liljequist
影响因子：
--
作者：
GIMENO, AL;GIMENO, MF;WEBB, JL
通讯作者：
WEBB, JL
乙醇对突触体和肝细胞细胞内离子钙浓度的影响。
影响因子：
3.6
作者：
Daniell,LC;Brass,EP;Harris,RA
通讯作者：
Harris,RA