Microfluidic paper-based analytical device for aerosol oxidative activity.
Microfluidic paper-based analytical device for aerosol oxidative activity.
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DOI：
10.1021/es304662w
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发表时间：
2013-01-15
影响因子：
11.4
通讯作者：
Volckens, John
中科院分区：
文献类型：
--
作者：
Sameenoi, Yupaporn;Panymeesamer, Pantila;Supalakorn, Natcha;Koehler, Kirsten;Chailapakul, Orawon;Henry, Charles S.;Volckens, John
人类暴露于颗粒物(PM)空气污染除了与各种癌症有关外，还与呼吸系统、心血管和神经退行性疾病有关。在所有这些关联中，一致的假设是PM在受影响的组织中诱导炎症和氧化应激。因此，已经开发了各种方法来量化PM的氧化活性，作为表征其诱导氧化应激能力的一种手段。由于检测灵敏度和检测限的限制，这些检测中的绝大多数依赖于大容量、固定位置的采样方法。因此，我们对个人暴露如何导致氧化空气污染的摄入量的理解是有限的。为了进一步了解这一点，我们提出了一种微流控纸基分析设备(μPAD)，用于测量通过个人采样收集的过滤器上的PM氧化活性。μ垫的制造成本低廉，可提供快速而灵敏的气溶胶氧化活性分析。氧化活性测定基于二硫苏糖醇测定法(DTT测定法)，采用比色法检测，可在采集样品后30分钟内现场完成。使用13个提取的气溶胶样品，包括城市气溶胶、生物质燃烧PM、香烟烟雾和香烟烟雾，验证了μPAD分析与传统的直接滴定试验。结果显示，两种方法测得的DTT消耗率无显著差异。为了证明这种方法的实用性，在科罗拉多州柯林斯堡，收集了个人样本，以估计人体从室内空气、晴朗日子的室外空气和受野火影响的一天的室外空气中暴露于PM的量。在大火当天收集的过滤样品在质量归一化的基础上显示出最高的氧化活性，而典型的环境背景空气显示出最低的氧化活性。
Human exposure to particulate matter (PM) air pollution has been linked with respiratory, cardiovascular, and neurodegenerative diseases, in addition to various cancers. Consistent among all of these associations is the hypothesis that PM induces inflammation and oxidative stress in the affected tissue. Consequently, a variety of assays have been developed to quantify the oxidative activity of PM as a means to characterize its ability to induced oxidative stress. The vast majority of these assays rely on high-volume, fixed-location sampling methods due to limitations in assay sensitivity and detection limit. As a result, our understanding of how personal exposure contributes to the intake of oxidative air pollution is limited. To further this understanding, we present a microfluidic paper-based analytical device (μPAD) for measuring PM oxidative activity on filters collected by personal sampling. The μPAD is inexpensive to fabricate and provides fast and sensitive analysis of aerosol oxidative activity. The oxidative activity measurement is based on the dithiothreitol assay (DTT assay), uses colorimetric detection, and can be completed in the field within 30 min following sample collection. The μPAD assay was validated against the traditional DTT assay using 13 extracted aerosol samples including urban aerosols, biomass burning PM, cigarette smoke and incense smoke. The results showed no significant differences in DTT consumption rate measured by the two methods. To demonstrate the utility of the approach, personal samples were collected to estimate human exposures to PM from indoor air, outdoor air on a clean day, and outdoor air on a wildfire-impacted day in Fort Collins, CO. Filter samples collected on the wildfire day gave the highest oxidative activity on a mass normalized basis, whereas typical ambient background air showed the lowest oxidative activity.
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影响因子：
10.4
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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