Alphacoronaviruses Are Common in Bats in the Upper Midwestern United States.
Alphacoronaviruses Are Common in Bats in the Upper Midwestern United States.
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DOI：
10.3390/v14020184
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发表时间：
2022-01-19
期刊：
影响因子：
--
通讯作者：
Hause BM
中科院分区：
文献类型：
--
作者：
Schaeffer R;Temeeyasen G;Hause BM
蝙蝠是冠状病毒（CoVs）的天然宿主，会周期性地传播给人类，严重急性呼吸综合征冠状病毒（SARS - CoV）和新冠病毒（SARS - CoV - 2）就是例证。对来自南达科他州、明尼苏达州、艾奥瓦州和内布拉斯加州的174份因人类接触而送检狂犬病病毒检测的蝙蝠样本，使用泛冠状病毒聚合酶链式反应（PCR）进行了分析。通过巢式逆转录PCR在12份（6.9%）样本中鉴定出一种先前已部分特征化的冠状病毒——棕蝠冠状病毒（Eptesicus bat CoV）。确定了6个近乎完整的基因组。基因分析发现所有冠状病毒阳性样本、落基山蝙蝠冠状病毒65以及近期在韩国发现的甲型冠状病毒HCQD - 2020之间具有高度相似性。对基因组序列的系统发育分析表明，棕蝠冠状病毒与蝙蝠冠状病毒HKU2和猪急性腹泻综合征冠状病毒密切相关；然而，注意到刺突基因存在拓扑不一致性，它与猪流行性腹泻病毒关系更密切。与一些甲型冠状病毒类似，在核衣壳下游发现了一个新基因ORF7，其编码的蛋白质与已知蛋白质缺乏相似性。棕蝠冠状病毒的广泛传播以及它与已传播给猪的蝙蝠病毒具有相似性，这值得进一步监测。
Bats are a reservoir for coronaviruses (CoVs) that periodically spill over to humans, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. A collection of 174 bat samples originating from South Dakota, Minnesota, Iowa, and Nebraska submitted for rabies virus testing due to human exposure were analyzed using a pan-coronavirus PCR. A previously partially characterized CoV, Eptesicus bat CoV, was identified in 12 (6.9%) samples by nested RT-PCR. Six near-complete genomes were determined. Genetic analysis found a high similarity between all CoV-positive samples, Rocky Mountain bat CoV 65 and alphacoronavirus HCQD-2020 recently identified in South Korea. Phylogenetic analysis of genome sequences showed EbCoV is closely related to bat CoV HKU2 and swine acute diarrhea syndrome CoV; however, topological incongruences were noted for the spike gene that was more closely related to porcine epidemic diarrhea virus. Similar to some alphaCoVs, a novel gene, ORF7, was discovered downstream of the nucleocapsid, whose protein lacked similarity to known proteins. The widespread circulation of EbCoV with similarities to bat viruses that have spilled over to swine warrants further surveillance.
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影响因子：
10.7
作者：
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通讯作者：
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DOI：
10.3390/v13040599
发表时间：
2021-04-01
期刊：
Viruses
影响因子：
--
作者：
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DOI：
10.1016/s0006-291x(03)01192-6
发表时间：
2003-07-25
影响因子：
3.1
作者：
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