Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration.
Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration.
复制标题
生物相容性纳米复合材料增强牙周膜干细胞的成骨和牙骨质分化，促进牙周再生
DOI：
10.3390/ma13214951
复制
发表时间：
2020-11-04
期刊：
影响因子：
--
通讯作者：
Xu HHK
中科院分区：
文献类型：
--
作者：
Liu J;Dai Q;Weir MD;Schneider A;Zhang C;Hack GD;Oates TW;Zhang K;Li A;Xu HHK
牙齿根部的腐烂在老年人中很普遍，因为人们寿命更长，并且保留更多的牙齿到老年，特别是牙周病和牙龈萎缩的患者。本研究的目的是开发一种具有生物相容性的纳米级氟化钙纳米复合材料为探讨纳米CaF 2对人牙周膜干细胞（hPDLSCs）成骨和成牙骨质诱导的影响，采用喷雾干燥法制备了平均粒径为53 nm的纳米CaF 2颗粒，并将纳米CaF 2以0%的质量分数掺入到复合材料中，10质量%、15质量%和20质量%。纳米CaF 2复合材料的弯曲强度（160 ± 10）MPa、弹性模量（11.0 ± 0.5）GPa、硬度（0.58 ± 0.03）GPa均高于市售牙科复合材料（p < 0.05）。钙（Ca）和氟（F）离子从复合材料中稳定释放。在20%纳米CaF 2上生长的hPDLSC的成骨基因升高。碱性磷酸酶（ALP）在14天达到高峰。胶原蛋白1（COL 1）、侏儒相关转录因子2（RUNX 2）和骨桥蛋白（OPN）在21天时达到峰值。20%纳米CaF 2复合材料促进牙骨质形成基因表达，促进牙骨质粘附蛋白（CAP）、牙骨质蛋白1（CEMP 1）和骨唾液蛋白（BSP）表达（p < 0.05）。在第7、14和21天，20% Nano-CaF 2复合物上的hPDLSC的ALP活性分别是对照的57倍、78倍和55倍（p < 0.05）。在20% Nano-CaF 2复合材料上的hPDLSC的骨矿物质分泌是对照的2倍（p < 0.05）。总之，新型Nano-CaF 2复合材料具有生物相容性并支持hPDLSC。纳米CaF 2复合材料有望填充牙根洞，释放Ca、F离子，促进hPDLSCs成骨、成牙骨质诱导，促进牙周组织再生。
Decays in the roots of teeth is prevalent in seniors as people live longer and retain more of their teeth to an old age, especially in patients with periodontal disease and gingival recession. The objectives of this study were to develop a biocompatible nanocomposite with nano-sized calcium fluoride particles (Nano-CaF2), and to investigate for the first time the effects on osteogenic and cementogenic induction of periodontal ligament stem cells (hPDLSCs) from human donors.Nano-CaF2 particles with a mean particle size of 53 nm were produced via a spray-drying machine.Nano-CaF2 was mingled into the composite at 0%, 10%, 15% and 20% by mass. Flexural strength (160 ± 10) MPa, elastic modulus (11.0 ± 0.5) GPa, and hardness (0.58 ± 0.03) GPa for Nano-CaF2 composite exceeded those of a commercial dental composite (p < 0.05). Calcium (Ca) and fluoride (F) ions were released steadily from the composite. Osteogenic genes were elevated for hPDLSCs growing on 20% Nano-CaF2. Alkaline phosphatase (ALP) peaked at 14 days. Collagen type 1 (COL1), runt-related transcription factor 2 (RUNX2) and osteopontin (OPN) peaked at 21 days. Cementogenic genes were also enhanced on 20% Nano-CaF2 composite, promoting cementum adherence protein (CAP), cementum protein 1 (CEMP1) and bone sialoprotein (BSP) expressions (p < 0.05). At 7, 14 and 21 days, the ALP activity of hPDLSCs on 20% Nano-CaF2 composite was 57-fold, 78-fold, and 55-fold greater than those of control, respectively (p < 0.05). Bone mineral secretion by hPDLSCs on 20% Nano-CaF2 composite was 2-fold that of control (p < 0.05). In conclusion, the novel Nano-CaF2 composite was biocompatible and supported hPDLSCs. Nano-CaF2 composite is promising to fill tooth root cavities and release Ca and F ions to enhance osteogenic and cementogenic induction of hPDLSCs and promote periodontium regeneration.
登录
查看更多内容
DOI：
10.1111/j.1754-4505.2012.00280.x
发表时间：
2012-11
期刊：
Special care in dentistry : official publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry
影响因子：
--
作者：
Kim JK;Baker LA;Seirawan H;Crimmins EM
通讯作者：
Crimmins EM
影响因子：
5.8
作者：
Anitua, Eduardo;Pinas, Laura;Tejero, Ricardo
通讯作者：
Tejero, Ricardo
影响因子：
2.4
作者：
Gofa, A;Davidson, RM
通讯作者：
Davidson, RM
影响因子：
4.3
作者：
Eke PI;Dye BA;Wei L;Slade GD;Thornton-Evans GO;Borgnakke WS;Taylor GW;Page RC;Beck JD;Genco RJ
通讯作者：
Genco RJ
影响因子：
--
作者：
Ayobian-Markazi N;Fourootan T;Kharazifar MJ
通讯作者：
Kharazifar MJ