Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis)
Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis)
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南方鲶 (Silurus meridionalis) 卵巢细胞系的建立和表征
DOI：
10.1007/s10695-014-9932-9
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发表时间：
2014-03
影响因子：
2.9
通讯作者：
Wang, Deshou
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoping;Dong, Ranran;Zhou, Linyan;Wang, Deshou
以南方鲇幼鱼卵巢为材料，成功建立了南方鲇卵巢细胞系SCO 1。该细胞系在28 °C下在含有15%胎牛血清的L-15培养基中优先增殖，在420天的时间内传代超过70代。SCO 1显示成纤维细胞样形态，主要保留了58条染色体的二倍体核型。从基因表达谱来看，SCO 1具有卵巢颗粒细胞的特征。用绿色荧光蛋白表达载体转染细胞后，在约30%的细胞中可以观察到明亮的荧光信号。该细胞系可能对内分泌干扰物的评价和研究体细胞和生殖细胞之间的相互作用有价值。
An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibroblast-like morphology and predominantly retained a diploid karyotype of 58 chromosomes. From the gene expression patterns, SCO1 showed a characteristic of ovarian granulosa cells. After the cells were transfected with the green fluorescent protein expression vector, bright fluorescent signals could be observed in approximately 30 % cells. This cell line may be valuable for the evaluation of endocrine disruptors and studying interactions between somatic cells and germ cells.
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影响因子：
2.5
作者：
V. S. Babu;K. Nambi;V. Chandra;V. P. Ishaq Ahmed;R. Bhonde;A. S. Sahul Hameed
通讯作者：
V. S. Babu;K. Nambi;V. Chandra;V. P. Ishaq Ahmed;R. Bhonde;A. S. Sahul Hameed
半滑舌鳎睾丸细胞系的建立和表征
影响因子：
9.2
作者：
Zhang B;Wang X;Sha Z;Yang C;Liu S;Wang N;Chen SL
通讯作者：
Chen SL
DOI：
10.1007/bf02618357
发表时间：
1980-05
期刊：
In Vitro
影响因子：
--
作者：
P. Bowser;J. Plumb
通讯作者：
P. Bowser;J. Plumb
DOI：
10.1016/j.cbpa.2008.12.013
发表时间：
2009-05
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
Fengrui Wu;Xiuyue Zhang;Weili Zhang;Baofeng Huang;Zhihao Liu;Chongjiang Hu;Deshou Wang
通讯作者：
Fengrui Wu;Xiuyue Zhang;Weili Zhang;Baofeng Huang;Zhihao Liu;Chongjiang Hu;Deshou Wang
影响因子：
2.9
作者：
S. Higaki;Yoshie Koyama;E. Shirai;T. Yokota;Y. Fujioka;Noriyoshi Sakai;T. Takada
通讯作者：
S. Higaki;Yoshie Koyama;E. Shirai;T. Yokota;Y. Fujioka;Noriyoshi Sakai;T. Takada