Fine-mapping of the woolly gene controlling multicellular trichome formation and embryonic development in tomato
Fine-mapping of the woolly gene controlling multicellular trichome formation and embryonic development in tomato
复制标题
控制番茄多细胞毛状体形成和胚胎发育的羊毛基因的精细定位
DOI：
10.1007/s00122-011-1612-x
复制
发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
李汉霞
中科院分区：
文献类型：
--
作者：
李汉霞
毛状体是一种来源于几乎所有陆地植物的表皮细胞的小毛，它们以单细胞和多细胞的形式存在。拟南芥中单细胞毛状体的调控途径已被很好地描述。然而，对番茄(Solanum Lycopsicum)多细胞毛的形成知之甚少。在番茄中，Woolly(Wo)基因控制多细胞毛状体的启动，并在纯合时导致胚胎死亡。为了克隆和鉴定Wo，利用基于图谱的克隆策略将该基因精细定位到一个约200kb的DNA片段上。本研究利用了一系列基于序列的分子标记，包括简单序列重复序列、序列特征扩增区和裂解扩增多态序列。对该序列的分析表明，该区域含有19个推测的开放阅读框。这些结果不仅为Wo的分离和鉴定提供了重要的信息，也为研究番茄毛状体形成和胚胎致死的调控途径奠定了基础。
Trichomes are small hairs that originate from the epidermal cells of nearly all land plants, and they exist in unicellular and multicellular forms. The regulatory pathway of unicellular trichomes in Arabidopsis is well characterized. However, little is known about the multicellular trichome formation in tomato (Solanum lycopersicum). The woolly (Wo) gene controls multicellular trichome initiation and leads to embryonic lethality when homozygous in tomato. To clone and characterize Wo, the gene was fine-mapped to a DNA fragment of ~200 kb using the map-based cloning strategy. A series of sequence-based molecular markers, including simple sequence repeat, sequence characterized amplified region, and cleaved amplified polymorphic sequence were utilized in this study. Analysis of the sequence indicated that this region carries 19 putative open reading frames. These results will provide not only the important information for the isolation and characterization of Wo but also the starting point for studying the regulatory pathway responsible for trichome formation and embryonic lethality in tomato.
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影响因子：
3.3
作者：
C. Payne;Fan Zhang;A. Lloyd
通讯作者：
C. Payne;Fan Zhang;A. Lloyd
影响因子：
4.6
作者：
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通讯作者：
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影响因子：
30.8
作者：
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通讯作者：
Tanksley, Steven D.
影响因子：
11.6
作者：
Walker, AR;Davison, PA;Gray, JC
通讯作者：
Gray, JC
DOI：
--
发表时间：
1959-09
期刊：
--
影响因子：
--
作者：
Paul R. Shilling
通讯作者：
Paul R. Shilling