A survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenya.
A survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenya.
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DOI：
10.1371/journal.pone.0252369
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Villinger J
中科院分区：
文献类型：
--
作者：
Chiuya T;Masiga DK;Falzon LC;Bastos ADS;Fèvre EM;Villinger J
埃及伊蚊和尖音库蚊复合体是虫媒病毒的多产载体，对人类和动物健康构成全球性威胁。全球化的发展和旅行的便利促进了这些蚊子及其传播的病毒在世界范围内的传播。为了评估疾病的风险，我们确定了在肯尼亚西部的县接壤的高虫媒病毒活动的地区的虫媒病毒的频率。除致病性病毒外，还评价了昆虫特异性黄病毒（ISF），其中一些被认为会损害特定致病性虫媒病毒的传播。我们在2018年和2019年的短雨季和长雨季在牲畜市场和医院捕捉蚊子。蚊子进行了筛选登革热，基孔肯雅和其他人类致病性虫媒病毒，ISF，和他们的血餐来源，确定高分辨率的（RT-）PCR产物的熔解分析。在收集到的6,848只蚊子中，89%是在漫长的雨季被诱捕的，A.埃及伊蚊（59%）和Cx.广义上的pipiens lato（40%）是最丰富的。大多数吸血蚊子是Cx。皮皮恩斯公司用来自人类、鸡和麻雀的血餐（Passer sp.）。我们没有检测到登革热或基孔肯雅病毒。但有1只多足库蚊雌蚊检出辛德毕斯病毒，30个Ae.埃及伊蚊有细胞融合剂病毒（CFAV，感染率（IR）= 1.27%，95% CI = 0.87%~ 1.78%）;埃及伊蚊有伊蚊黄病毒（AeFV; IR = 0.43%，95%CI = 0.23%-0.74%）;皮皮恩斯公司(IR= 0.23%，95%CI = 0.1%-0.45%），一个库的库蚊黄病毒。辛德毕斯病毒可引起人类发热性疾病，可使发热患者的诊断和预后复杂化。在一个单一的蚊子与ISF蚊子种群的辛德毕斯病毒的存在，要求进一步调查ISF可能发挥的作用，在该地区阻断其他虫媒病毒的传播。
Aedes aegypti and Culex pipiens complex mosquitoes are prolific vectors of arboviruses that are a global threat to human and animal health. Increased globalization and ease of travel have facilitated the worldwide dissemination of these mosquitoes and the viruses they transmit. To assess disease risk, we determined the frequency of arboviruses in western Kenyan counties bordering an area of high arboviral activity. In addition to pathogenic viruses, insect-specific flaviviruses (ISFs), some of which are thought to impair the transmission of specific pathogenic arboviruses, were also evaluated. We trapped mosquitoes in the short and long rainy seasons in 2018 and 2019 at livestock markets and hospitals. Mosquitoes were screened for dengue, chikungunya and other human pathogenic arboviruses, ISFs, and their blood-meal sources as determined by high-resolution melting analysis of (RT-)PCR products. Of 6,848 mosquitoes collected, 89% were trapped during the long rainy season, with A. aegypti (59%) and Cx. pipiens sensu lato (40%) being the most abundant. Most blood-fed mosquitoes were Cx. pipiens s.l. with blood-meals from humans, chicken, and sparrow (Passer sp.). We did not detect dengue or chikungunya viruses. However, one Culex poicilipes female was positive for Sindbis virus, 30 pools of Ae. aegypti had cell fusing agent virus (CFAV; infection rate (IR) = 1.27%, 95% CI = 0.87%-1.78%); 11 pools of Ae. aegypti had Aedes flavivirus (AeFV; IR = 0.43%, 95% CI = 0.23%-0.74%); and seven pools of Cx. pipiens s.l. (IR = 0.23%, 95% CI = 0.1%-0.45%) and one pool of Culex annulioris had Culex flavivirus. Sindbis virus, which causes febrile illness in humans, can complicate the diagnosis and prognosis of patients with fever. The presence of Sindbis virus in a single mosquito from a population of mosquitoes with ISFs calls for further investigation into the role ISFs may play in blocking transmission of other arboviruses in this region.
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3.7
作者：
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通讯作者：
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3.2
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