CD44v/CD44s expression patterns are associated with the survival of pancreatic carcinoma patients.
CD44v/CD44s expression patterns are associated with the survival of pancreatic carcinoma patients.
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CD44v/CD44s 表达模式与胰腺癌患者的生存相关
DOI：
10.1186/1746-1596-9-79
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发表时间：
2014-04-08
影响因子：
2.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Li Z;Chen K;Jiang P;Zhang X;Li X;Li Z
背景与目的CD44变异与肿瘤侵袭和转移有关，但CD44在胰腺癌中的表达模式尚未得到系统的研究。本研究系统探讨了CD44表达模式是否与胰腺癌转移及预后有关。方法采用实时荧光定量PCR （qRT-PCR）技术，对CD44 （CD44v2-CD44v10和CD44s）的表达模式进行分析。然后我们通过临床生存分析进一步评估它们在胰腺癌转移和预后中的作用。结果3种不同细胞系及人肿瘤组织中CD44v表达升高，CD44s表达降低。临床分析显示CD44v6+、CD44v9+与淋巴结转移、肝转移及TNM分期相关。然而，CD44s−与肝转移、肿瘤分化和TNM分期有关。生存分析显示，CD44v6+/CD44s -或CD44v6+/CD44s -患者的总生存率（OS）较低，尽管CD44v6、CD44v9和CD44s的个体表达也与OS率降低有关。单因素分析显示淋巴结转移；船入侵;肝转移;TNM阶段;CD44v6、CD44v9和CD44s的单独或共表达是影响生存的危险因素。多变量分析显示，CD44v6+/CD44s−是生存的独立预测因子。结论我们发现CD44v6+、CD44v9+和CD44s -与胰腺癌的转移和进展有关，CD44v6+/CD44s -是影响胰腺癌生存的独立危险因素。因此，CD44v/CD44s的不同表达模式可能决定胰腺癌的预后。虚拟幻灯片本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/1579257224116287。
Background and purposeCD44 variants have been associated with tumor invasion and metastasis, but CD44 expression patterns have not been systematically investigated in pancreatic carcinoma. This study systematically investigated whether CD44 expression patterns are involved in pancreatic carcinoma metastasis and prognosis.MethodsWe applied primers specific for all CD44 variants and CD44s to analyze the expression patterns of CD44 (CD44v2-CD44v10 and CD44s) using quantitative real-time PCR (qRT-PCR). We then further evaluated their roles in pancreatic carcinoma metastasis and prognosis using clinical survival analysis.ResultsIncreased CD44v expression and decreased CD44s expression were found in metastatic pancreatic carcinoma in three different cell lines and in human tumor tissue. Clinical analysis showed that CD44v6+and CD44v9+were correlated with lymph node metastasis, liver metastasis and TNM stage. However, CD44s−was associated with liver metastasis, tumor differentiation and TNM stage. Survival analysis showed that patients with CD44v6+/CD44s−or CD44v6+/CD44s−had lower overall survival (OS) rates, although the individual expression of CD44v6, CD44v9 and CD44s was also related to decreased OS rates. Univariate analysis showed that lymph node metastasis; vessel invasion; hepatic metastases; TNM stage; and individual or co-expression of CD44v6, CD44v9 and CD44s were risk factors affecting survival. Multivariate analysis showed that CD44v6+/CD44s−was an independent predictor of survival.ConclusionsWe found that CD44v6+, CD44v9+and CD44s−were associated with pancreatic carcinoma metastasis and progression and that CD44v6+/CD44s−was an independent risk factor affecting survival in pancreatic carcinoma. Therefore, the different expression patterns of CD44v/CD44s may determine pancreatic carcinoma prognosis.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1579257224116287 .
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影响因子：
8.8
作者：
Maeda, S.;Shinchi, H.;Kurahara, H.;Mataki, Y.;Noma, H.;Maemura, K.;Aridome, K.;Yokomine, T.;Natsugoe, S.;Aikou, T.;Takao, S.
通讯作者：
Takao, S.
影响因子：
64.8
作者：
GALLATIN, WM;WEISSMAN, IL;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
2.6
作者：
Zhou G;Chiu D;Qin D;Niu L;Cai J;He L;Tan D;Xu K
通讯作者：
Xu K
影响因子：
50.3
作者：
Ishimoto, Takatsugu;Nagano, Osamu;Saya, Hideyuki
通讯作者：
Saya, Hideyuki
DOI：
10.1097/pai.0b013e318047df6d
发表时间：
2008-03-01
影响因子：
1.6
作者：
Afify, Alaa;McNiel, Maaya A.;Paulino, Augusto F.
通讯作者：
Paulino, Augusto F.