Wastewater surveillance of Mpox virus in Baltimore.
Wastewater surveillance of Mpox virus in Baltimore.
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DOI：
10.1016/j.scitotenv.2023.164414
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发表时间：
2023-09-15
影响因子：
9.8
通讯作者：
Thakali, Ocean
中科院分区：
文献类型：
--
作者：
Sherchan, Samendra P.;Solomon, Tamunobelema;Idris, Oladele;Nwaubani, Daniel;Thakali, Ocean
本研究旨在利用废水监测在社区一级监测m痘病例。从2022年7月27日至2022年9月22日，从巴尔的摩市的两个污水处理厂（a和B）每周收集一次未经处理的废水样本。采用吸附-洗脱（AE）法和聚乙二醇（PEG）沉淀法对样品进行浓缩，然后进行定量聚合酶链反应（qPCR）。用至少一种浓度法在污水处理厂A区89%（8/9）和B区55%（5/9）的样本中检出猴痘病毒（MPXV）。与AE法相比，聚乙二醇沉淀法对MPXV的检出率更高，表明聚乙二醇沉淀法是一种更有效的病毒浓缩方法。据我们所知，这是第一个报道在巴尔的摩废水中检测MPXV的研究。这些结果强调，废水监测可以作为监测未来Mpox暴发的补充预警工具。
This study aimed to utilize wastewater surveillance for monitoring Mpox cases at a community level. Untreated wastewater samples were collected once a week from two wastewater treatment plants (A and B) in Baltimore City from July 27, 2022–September 22, 2022. The samples were concentrated via an adsorption–elution (AE) method and Polyethylene Glycol (PEG) precipitation method followed by quantitative polymerase chain reaction (qPCR). Monkeypox virus (MPXV) was detected in 89 % (8/9) samples from WWTP A and 55 % (5/9) samples from WWTP B with at least one concentration method. Higher detection rate in samples concentrated with PEG precipitation compared to AE method was observed, indicating that PEG precipitation is a more effective virus concentration method for MPXV. To our knowledge, this is the first study reporting the detection of MPXV in wastewater in Baltimore. The results highlight that wastewater surveillance could be used as a complementary early warning tool for monitoring future Mpox outbreaks.
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