In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize.
In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize.
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DOI：
10.1186/1754-6834-4-1
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发表时间：
2011-01-26
影响因子：
6.3
通讯作者：
Decker SR
中科院分区：
文献类型：
--
作者：
Brunecky R;Selig MJ;Vinzant TB;Himmel ME;Lee D;Blaylock MJ;Decker SR
来自 Acidothermus cellulolyticus 的糖苷水解酶家族 5 内切纤维素酶 E1 (Cel5A) 被转化到烟草和玉米中，并在组成型启动子的作用下靶向细胞壁表达。在这里，我们探讨了在植物中表达内切纤维素酶将允许这些酶在细胞壁构建过程中接触其底物的可能性，从而使纤维素更适合预处理和酶消化。与野生型（WT）相比，烟草和玉米植株健康且发育正常。经过热化学预处理和酶消化后，转化植物明显比 WT 更容易消化，需要较低的预处理强度才能达到相当的转化水平。此外，不顺应性的降低并不是由于预处理后残留的E1活性，并且不能通过在预处理之前向生物量中添加外源E1来重现，这表明细胞壁构建过程中E1的表达改变了细胞壁的固有逆顺性。
The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a constitutive promoter. Here we explore the possibility that in planta expression of endocellulases will allow these enzymes to access their substrates during cell wall construction, rendering cellulose more amenable to pretreatment and enzyme digestion. Tobacco and maize plants were healthy and developed normally compared with the wild type (WT). After thermochemical pretreatment and enzyme digestion, transformed plants were clearly more digestible than WT, requiring lower pretreatment severity to achieve comparable conversion levels. Furthermore, the decreased recalcitrance was not due to post-pretreatment residual E1 activity and could not be reproduced by the addition of exogenous E1 to the biomass prior to pretreatment, indicating that the expression of E1 during cell wall construction altered the inherent recalcitrance of the cell wall.
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影响因子：
3.8
作者：
Donohoe, Bryon S.;Selig, Michael J.;Himmel, Michael E.
通讯作者：
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影响因子：
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Decker, Stephen R.
影响因子：
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影响因子：
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