Projections of nucleus accumbens adenosine A2A receptor neurons in the mouse brain and their implications in mediating sleep-wake regulation.
Projections of nucleus accumbens adenosine A2A receptor neurons in the mouse brain and their implications in mediating sleep-wake regulation.
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小鼠大脑中伏隔核腺苷 A2A 受体神经元的投射及其在介导睡眠-觉醒调节中的意义
DOI：
10.3389/fnana.2013.00043
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发表时间：
2013
影响因子：
2.9
通讯作者：
Li RX
中科院分区：
文献类型：
--
作者：
Zhang JP;Xu Q;Yuan XS;Cherasse Y;Schiffmann SN;de Kerchove d'Exaerde A;Qu WM;Urade Y;Lazarus M;Huang ZL;Li RX
伏隔核 (Acb) 中的腺苷 A2A 受体 (A2AR) 已被证明在腺苷受体拮抗剂咖啡因的唤醒作用中发挥重要作用，并可能参与生理性睡眠。为了更好地了解这些受体在睡眠中的功能，利用编码人源化海肾绿色荧光蛋白（hrGFP）的腺相关病毒（AAV）作为长轴突通路的示踪剂绘制了 A2AR 神经元的投影图。将 Cre 依赖性 AAV 注射到 A2AR-Cre 小鼠中 Acb 的核心 (AcbC) 和外壳 (AcbSh) 中。然后使用免疫组织化学可视化 hrGFP，突出显示注射部位 A2AR 神经元的核周及其投影区域的轴突。数据显示，A2AR 神经元表现出中等大小的圆形或椭圆形周核，其突起位于 Acb 内。此外，Acb 的投射分布到前脑、间脑和脑干的细胞核。在前脑中，来自所有 Acb 亚区的 A2AR 神经元共同投射到腹侧苍白球、对角带核和无名质。在外侧下丘脑和外侧视前区观察到来自 AcbC 和腹侧 AcbSh 的强烈投射以及来自内侧 AcbSh 的较弱投射。在脑干中，Acb 投射位于腹侧被盖区，而 AcbC 和腹侧 AcbSh 也投射到中缝核、中缝背核和腹外侧导水管周围灰质。这些结果为理解 Acb 中 A2AR 和 A2AR 神经元的作用，特别是在睡眠调节中的作用提供了坚实的基础。
Adenosine A2A receptors (A2ARs) in the nucleus accumbens (Acb) have been demonstrated to play an important role in the arousal effect of adenosine receptor antagonist caffeine, and may be involved in physiological sleep. To better understand the functions of these receptors in sleep, projections of A2AR neurons were mapped utilizing adeno-associated virus (AAV) encoding humanized Renilla green fluorescent protein (hrGFP) as a tracer for long axonal pathways. The Cre-dependent AAV was injected into the core (AcbC) and shell (AcbSh) of the Acb in A2AR-Cre mice. Immunohistochemistry was then used to visualize hrGFP, highlighting the perikarya of the A2AR neurons in the injection sites, and their axons in projection regions. The data revealed that A2AR neurons exhibit medium-sized and either round or elliptic perikarya with their processes within the Acb. Moreover, the projections from the Acb distributed to nuclei in the forebrain, diencephalon, and brainstem. In the forebrain, A2AR neurons from all Acb sub-regions jointly projected to the ventral pallidum, the nucleus of the diagonal band, and the substantia innominata. Heavy projections from the AcbC and the ventral AcbSh, and weaker projections from the medial AcbSh, were observed in the lateral hypothalamus and lateral preoptic area. In the brainstem, the Acb projections were found in the ventral tegmental area, while AcbC and ventral AcbSh also projected to the median raphe nucleus, the dorsal raphe nucleus, and the ventrolateral periaqueductal gray. The results supply a solid base for understanding the roles of the A2AR and A2AR neurons in the Acb, especially in the regulation of sleep.
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DOI：
10.1016/0169-328x(92)90173-9
发表时间：
1992-07-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
FINK, JS;WEAVER, DR;REPPERT, SM
通讯作者：
REPPERT, SM
影响因子：
3.8
作者：
BARRACO, RA;MARTENS, KA;NORMILE, HJ
通讯作者：
NORMILE, HJ
影响因子：
2.5
作者：
GROENEWEGEN, HJ;RUSSCHEN, FT
通讯作者：
RUSSCHEN, FT
影响因子：
25
作者：
Huang, ZL;Qu, WM;Hayaishi, O
通讯作者：
Hayaishi, O
影响因子：
3.4
作者：
JONGENRELO, AL;VOORN, P;GROENEWEGEN, HJ
通讯作者：
GROENEWEGEN, HJ