Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress.
Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress.
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在冷应激下的Shewanella Putrefaciens WS13生物膜的比较转录组分析。
DOI：
10.3389/fcimb.2022.851521
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
腐败希瓦氏杆菌是一种革兰氏阴性细菌，在低温条件下会导致海鲜变质。这种细菌很容易形成生物膜，以增强其在具有挑战性的环境中的生存。我们先前的研究表明，腐败链霉菌WS13在低温(4℃)下形成的生物膜比在最适生长温度(30℃)下形成的生物量更大，结构更紧密。在本研究中，利用Illumina RNA测序技术对腐败链霉菌WS13在冷藏和最适温度下形成的生物膜中基因表达的全球水平进行了比较转录组分析。结果表明，共有761个基因得到差异表达，其中497个基因显著上调，264个基因显著下调(p<；0.05)。随机选择的差异表达基因(Deg)的qRT-PCR结果证实了RNA测序结果。转录组数据的比较显示，在低温胁迫下，腐败链霉菌WS13的28条代谢途径发生了显著变化，包括下调的趋化性和运动性，上调的色氨酸代谢、组氨酸生物合成和群体感应，这些都有利于腐败链霉菌WS13生物膜的形成。本研究为更好地了解腐生链霉菌的生物被膜形成提供了有用的数据，也为针对低温胁迫下严重腐败菌的新型疫苗和药物靶点奠定了理论基础。
Shewanella putrefaciens is a Gram-negative bacterium that can cause seafood spoilage under low-temperature conditions. The bacterium easily forms biofilms to enhance its survival in challenging environments. Our previous research revealed that the biofilm formed by S. putrefaciens WS13 under the low temperature (4 °C) has larger biomass and tighter structure than at an optimum growth temperature (30 °C). In this study, comparative transcriptome analysis was further performed to get insights into the global-level of gene expression in the biofilm formed by S. putrefaciens WS13 under the refrigerating and optimal temperatures using Illumina RNA-Sequencing technique. The results revealed that a total of 761 genes were differentially expressed, of which 497 were significantly up-regulated and 264 were significantly down-regulated (p<0.05). The qRT-PCR results of randomly selected differentially expressed genes (DEGs) confirmed the RNA sequencing results. Comparison of transcriptome data revealed 28 significantly changed metabolic pathways under the cold stress, including the down-regulated chemotaxis, and motility, and up-regulated tryptophan metabolism, histidine biosynthesis, and quorum sensing, which benefited the biofilm formation of S. putrefaciens WS13 under the adverse circumstance. This study provided useful data for better understanding of the biofilm formation of S. putrefaciens, and also laid a theoretical foundation for novel vaccine and drug targets against the severe spoilage bacterium under the cold stress.
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影响因子：
9.2
作者：
Castro J;França A;Bradwell KR;Serrano MG;Jefferson KK;Cerca N
通讯作者：
Cerca N
丙酮丁醇梭菌生物膜和浮游细胞的比较转录组分析
影响因子：
4.1
作者：
Liu, Dong;Xu, Jiahui;Ying, Hanjie
通讯作者：
Ying, Hanjie
影响因子：
3.8
作者：
Alsaker, Keith V.;Paredes, Carlos;Papoutsakis, Eleftherios T.
通讯作者：
Papoutsakis, Eleftherios T.
影响因子：
5.4
作者：
Gram, L;Ravn, L;Givskov, M
通讯作者：
Givskov, M
影响因子：
4.4
作者：
Hamilton S;Bongaerts RJ;Mulholland F;Cochrane B;Porter J;Lucchini S;Lappin-Scott HM;Hinton JC
通讯作者：
Hinton JC