Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes.

Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes.
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DOI:
10.1007/s11468-012-9385-x
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发表时间:
2013-03
期刊:
影响因子:
3
通讯作者:
Di Fabrizio, Enzo
Di Fabrizio, Enzo
中科院分区:
物理与天体物理3区
文献类型:
--
作者:
Moretti, Manola;Zaccaria, Remo Proietti;Descrovi, Emiliano;Das, Gobind;Leoncini, Marco;Liberale, Carlo;De Angelis, Francesco;Di Fabrizio, Enzo

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Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.
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