Genomic imprinted genes in reciprocal hybrid endosperm of Brassica napus.
Genomic imprinted genes in reciprocal hybrid endosperm of Brassica napus.
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甘蓝型油菜互杂杂交胚乳中的基因组印记基因
DOI：
10.1186/s12870-021-02908-8
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发表时间：
2021-03-16
影响因子：
5.3
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Rong H;Yang W;Zhu H;Jiang B;Jiang J;Wang Y
基因组印记导致后代中亲本起源特异性等位基因的表达。甘蓝型油菜是一种具有多倍体研究价值的油料作物。甘蓝型油菜基因组印迹基因的鉴定将丰富双子叶植物基因组印迹的知识。本研究以甘蓝型油菜品种杨油6号（Y6）和中爽11号（ZS11）为材料，在授粉后20和25天采集胚乳（DAP）进行rna测序。根据Y6和ZS11之间的snp，共鉴定出297个印迹基因，包括283个母系表达基因（MEGs）和14个父系表达基因（peg）。只有36个基因（35个MEGs和1个PEG）在20和25个DAP胚乳中连续印迹。在拟南芥、水稻、蓖麻、玉米、油菜和其他油菜品系中分别发现了15个、2个、5个、3个、10个和25个印迹基因。只有26个印迹基因在甘蓝型油菜胚乳中特异表达，其余印迹基因在甘蓝型油菜根、茎、叶和花蕾中均有表达。共有109个印迹基因聚集在油菜染色体上。我们发现LTR/Copia转座因子（te）在印迹基因的上游和下游富集最多，并且在印迹基因周围富集的te多于非印迹基因。与亲本胚乳相比，杂交胚乳中5个agl基因和6个果胶相关基因的表达发生了显著变化。本研究对甘蓝型油菜的印迹基因进行了全面鉴定，丰富了双子叶植物的基因印迹，为进一步研究基因印迹如何调控种子发育提供了依据。在线版本包含补充资料，下载地址：10.1186/s12870-021-02908-8。
Genomic imprinting results in the expression of parent-of-origin-specific alleles in the offspring. Brassica napus is an oil crop with research values in polyploidization. Identification of imprinted genes in B. napus will enrich the knowledge of genomic imprinting in dicotyledon plants. In this study, we performed reciprocal crosses between B. napus L. cultivars Yangyou 6 (Y6) and Zhongshuang 11 (ZS11) to collect endosperm at 20 and 25 days after pollination (DAP) for RNA-seq. In total, we identified 297 imprinted genes, including 283 maternal expressed genes (MEGs) and 14 paternal expressed genes (PEGs) according to the SNPs between Y6 and ZS11. Only 36 genes (35 MEGs and 1 PEG) were continuously imprinted in 20 and 25 DAP endosperm. We found 15, 2, 5, 3, 10, and 25 imprinted genes in this study were also imprinted in Arabidopsis, rice, castor bean, maize, B. rapa, and other B. napus lines, respectively. Only 26 imprinted genes were specifically expressed in endosperm, while other genes were also expressed in root, stem, leaf and flower bud of B. napus. A total of 109 imprinted genes were clustered on rapeseed chromosomes. We found the LTR/Copia transposable elements (TEs) were most enriched in both upstream and downstream of the imprinted genes, and the TEs enriched around imprinted genes were more than non-imprinted genes. Moreover, the expression of 5 AGLs and 6 pectin-related genes in hybrid endosperm were significantly changed comparing with that in parent endosperm. This research provided a comprehensive identification of imprinted genes in B. napus, and enriched the gene imprinting in dicotyledon plants, which would be useful in further researches on how gene imprinting regulates seed development. The online version contains supplementary material available at 10.1186/s12870-021-02908-8.
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影响因子：
9.5
作者：
Bauer MJ;Fischer RL
通讯作者：
Fischer RL
影响因子：
11.6
作者：
Borisjuk, Ljudmilla;Neuberger, Thomas;Rolletschek, Hardy
通讯作者：
Rolletschek, Hardy
DOI：
10.1073/pnas.1019273108
发表时间：
2011-02-01
影响因子：
11.1
作者：
Hsieh, Tzung-Fu;Shin, Juhyun;Fischer, Robert L.
通讯作者：
Fischer, Robert L.
影响因子：
56.9
作者：
Grossniklaus, U;Vielle-Calzada, JP;Gagliano, WB
通讯作者：
Gagliano, WB
影响因子：
64.8
作者：
BARTOLOMEI, MS;ZEMEL, S;TILGHMAN, SM
通讯作者：
TILGHMAN, SM