Characterization of hepatitis B virus capsids by resistive-pulse sensing.
Characterization of hepatitis B virus capsids by resistive-pulse sensing.
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DOI：
10.1021/ja108228x
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发表时间：
2011-02-16
影响因子：
15
通讯作者：
Jacobson SC
中科院分区：
文献类型：
--
作者：
Zhou K;Li L;Tan Z;Zlotnick A;Jacobson SC
我们报道了通过在聚对苯二甲酸乙二酯膜中形成的单一轨迹刻蚀的锥形纳米孔的阻性脉冲传感来表征乙肝病毒衣壳的特征。孔的顶端直径约为40 nm，孔表面用三甘醇共价修饰，以降低表面电荷密度，最大限度地减少对病毒衣壳的吸附，并抑制孔内的电渗流。由乙肝病毒衣壳蛋白的组装结构域Cp149在体外组装了乙肝病毒衣壳。组装的衣壳T=3(90Cp149二聚体)和T=4(120二聚体)的直径分别为31和36 nm，通过监测衣壳通过电偏压孔时的电流变化很容易区分。穿过一个孔的T=3与T=4衣壳的数量之比不能反映实际浓度，但有利于较小的T=3衣壳的运输。这些结果与T=4衣壳较长的通过时间相结合，表明衣壳必须克服熵障碍才能进入气孔。
We report characterization of Hepatitis B virus (HBV) capsids by resistive-pulse sensing through single track-etched conical nanopores formed in poly(ethylene terephthalate) membranes. The pores were ~40 nm in diameter at the tip, and the pore surface was covalently modified with triethylene glycol to reduce surface charge density, minimize adsorption of the virus capsids, and suppress electroosmotic flow in the pore. The HBV capsids were assembled in vitro from Cp149, the assembly domain of HBV capsid protein. Assembled T=3 (90 Cp149 dimer) and T=4 (120 dimer) capsids are 31 and 36 nm in diameter, respectively, and were easily discriminated by monitoring the change in current as capsids passed through an electrically biased pore. The ratio of the number of T=3 to T=4 capsids transiting a pore did not reflect actual concentrations, but favored transport of smaller T=3 capsids. These results combined with longer transit times for the T=4 capsids indicated that the capsids must overcome an entropic barrier to enter a pore.
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DOI：
10.1016/s0168-583x(01)00722-4
发表时间：
2001-11-01
影响因子：
1.3
作者：
Apel, PY;Korchev, YE;Yoshida, M
通讯作者：
Yoshida, M
影响因子：
10.8
作者：
Heins, EA;Siwy, ZS;Martin, CR
通讯作者：
Martin, CR
影响因子：
1.6
作者：
DEBLOIS, RW;BEAN, CP
通讯作者：
BEAN, CP
影响因子：
5.7
作者：
Goldsmith, Jacob;Martens, Craig C.
通讯作者：
Martens, Craig C.
影响因子：
8.6
作者：
Han, J;Turner, SW;Craighead, HG
通讯作者：
Craighead, HG