Wolbachia Strain wGri From the Tea Geometrid Moth Ectropis grisescens Contributes to Its Host's Fecundity.
Wolbachia Strain wGri From the Tea Geometrid Moth Ectropis grisescens Contributes to Its Host's Fecundity.
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来自茶尺蛾 Ectropis grisescens 的 Wolbachia 菌株 wGri 有助于其宿主的繁殖力
DOI：
10.3389/fmicb.2021.694466
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发表时间：
2021
影响因子：
5.2
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Liu S;Jiang R;Zhang C;Gao T;Wang Y;Liu C;Long Y;Zhang Y;Yang Y
Members of the Wolbachia genus manipulate insect–host reproduction and are the most abundant bacterial endosymbionts of insects. The tea Geometrid moth Ectropis grisescens (Warren) (Lepidoptera: Geometridae) is the most devastating insect pest of tea plants [Camellia sinensis (L.) O. Kuntze] in China. However, limited data on the diversity, typing, or phenotypes of Wolbachia in E. grisescens are available. Here, we used a culture-independent method to compare the gut bacteria of E. grisescens and other tea Geometridae moths. The results showed that the composition of core gut bacteria in larvae of the three Geometridae moth species was similar, except for the presence of Wolbachia. Moreover, Wolbachia was also present in adult female E. grisescens samples. A Wolbachia strain was isolated from E. grisescens and designated as wGri. Comparative analyses showed that this strain shared multilocus sequence types and Wolbachia surface protein hypervariable region profiles with cytoplasmic incompatibility (CI)-inducing strains in supergroup B; however, the wGri-associated phenotypes were undetermined. A reciprocal cross analysis showed that Wolbachia-uninfected females mated with infected males resulted in 100% embryo mortality (0% eggs hatched per female). Eggs produced by mating between uninfected males and infected females hatched normally. These findings indicated that wGri induces strong unidirectional CI in E. grisescens. Additionally, compared with uninfected females, Wolbachia-infected females produced approximately 30–40% more eggs. Together, these results show that this Wolbachia strain induces reproductive CI in E. grisescens and enhances the fecundity of its female host. We also demonstrated that wGri potential influences reproductive communication between E. grisescens and Ectropis obliqua through CI.
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影响因子：
30.3
作者：
Kaur R;Shropshire JD;Cross KL;Leigh B;Mansueto AJ;Stewart V;Bordenstein SR;Bordenstein SR
通讯作者：
Bordenstein SR
影响因子：
6.4
作者：
Moriyama M;Nikoh N;Hosokawa T;Fukatsu T
通讯作者：
Fukatsu T
影响因子：
11
作者：
Ju, Jia-Fei;Bing, Xiao-Li;Hong, Xiao-Yue
通讯作者：
Hong, Xiao-Yue
DOI：
10.1073/pnas.101304298
发表时间：
2001-05-22
影响因子：
11.1
作者：
Dedeine, F;Vavre, F;Boulétreau, M
通讯作者：
Boulétreau, M
影响因子：
2.7
作者：
CHAO, A;MA, MC;YANG, MCK
通讯作者：
YANG, MCK