Acute ethanol treatment upregulates Th1, Th2, and Hdc in larval zebrafish in stable networks.
Acute ethanol treatment upregulates Th1, Th2, and Hdc in larval zebrafish in stable networks.
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急性乙醇处理在稳定网络中的幼虫斑马鱼中上调Th1，Th2和HDC。
DOI：
10.3389/fncir.2013.00102
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发表时间：
2013
影响因子：
3.5
通讯作者：
Panula P
中科院分区：
文献类型：
--
作者：
Puttonen HA;Sundvik M;Rozov S;Chen YC;Panula P
早期对斑马鱼的研究表明，急性给予乙醇对仔鱼的运动有刺激作用，但这种作用的机制尚未揭示。研究了乙醇浓度在0.75%~ 3.00%之间对7日龄图尔库品系斑马鱼幼鱼的影响。在0.75-3%浓度下，乙醇在第一分钟内增加游泳速度。在3%时，游泳速度在第一分钟后迅速下降，而在0.75和1.5%时，游泳速度延长。在最高浓度的乙醇多巴胺水平显着下降后，10分钟的治疗。我们发现，乙醇上调参与组胺（hdc）和多巴胺（th 1和th 2）的生物合成的关键基因后，短暂的10分钟乙醇处理，通过qPCR测量。使用原位杂交和免疫组织化学，我们进一步发现，形态的组胺能和多巴胺能神经元和网络的幼虫斑马鱼大脑是不受影响的10分钟和更长的30分钟的治疗。结果表明，急性乙醇迅速降低多巴胺水平，并激活这两种形式或TH补充多巴胺商店在30分钟内。组胺能和多巴胺能系统酶的动态变化发生在相同的细胞，通常表达的成绩单。由于已知多巴胺和组胺参与乙醇和自发刺激的行为效应，这些结果表明，这些网络的快速适应与改变的自发活动有关。
Earlier studies in zebrafish have revealed that acutely given ethanol has a stimulatory effect on locomotion in fish larvae but the mechanism of this effect has not been revealed. We studied the effects of ethanol concentrations between 0.75 and 3.00% on 7-day-old larval zebrafish (Danio rerio) of the Turku strain. At 0.75-3% concentrations ethanol increased swimming speed during the first minute. At 3% the swimming speed decreased rapidly after the first minute, whereas at 0.75 and 1.5% a prolonged increase in swimming speed was seen. At the highest ethanol concentration dopamine levels decreased significantly after a 10-min treatment. We found that ethanol upregulates key genes involved in the biosynthesis of histamine (hdc) and dopamine (th1 and th2) following a short 10-min ethanol treatment, measured by qPCR. Using in situ hybridization and immunohistochemistry, we further discovered that the morphology of the histaminergic and dopaminergic neurons and networks in the larval zebrafish brain was unaffected by both the 10-min and a longer 30-min treatment. The results suggest that acute ethanol rapidly decreases dopamine levels, and activates both forms or th to replenish the dopamine stores within 30 min. The dynamic changes in histaminergic and dopaminergic system enzymes occurred in the same cells which normally express the transcripts. As both dopamine and histamine are known to be involved in the behavioral effects of ethanol and locomotor stimulation, these results suggest that rapid adaptations of these networks are associated with altered locomotor activity.
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影响因子：
3.4
作者：
MacPhail, R. C.;Brooks, J.;Padilla, S.
通讯作者：
Padilla, S.
DOI：
10.1006/bbrc.2001.5511
发表时间：
2001-09-07
影响因子：
3.1
作者：
Dasmahapatra, AK;Doucet, HL;Carvan, MJ
通讯作者：
Carvan, MJ
影响因子：
2.8
作者：
Lee, YK;Park, SW;Kim, YH
通讯作者：
Kim, YH
影响因子：
3.4
作者：
Eriksson, KS;Peitsaro, N;Panula, P
通讯作者：
Panula, P
影响因子：
3.3
作者：
Farrar, A. M.;Segovia, K. N.;Salamone, J. D.
通讯作者：
Salamone, J. D.