Modulation of temporomandibular joint nociception and inflammation in male rats after administering a physiological concentration of 17β-oestradiol.
Modulation of temporomandibular joint nociception and inflammation in male rats after administering a physiological concentration of 17β-oestradiol.
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DOI：
10.1002/j.1532-2149.2012.00183.x
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发表时间：
2013-02
影响因子：
3.6
通讯作者：
Bellinger, L. L.
中科院分区：
文献类型：
--
作者：
Kramer, P. R.;Bellinger, L. L.
以往的研究表明，17β-雌二醇可以减轻雌性大鼠颞下颌关节（TMJ）的炎症和超敏反应。尽管雄性大鼠含有大量雌二醇，但尚不清楚生理浓度的17β-雌二醇是否会减弱雄性TMJ炎症和伤害感受。给予完整和去势大鼠生理浓度的雌二醇，首先检查雌二醇是否会影响雄性TMJ伤害感受/炎症，其次检查雌二醇给药是否会与内源性雄性激素协同作用以减弱TMJ伤害感受。给予经口治疗的大鼠TMJ注射完全弗氏佐剂（CFA），然后使用经验证的方法测量伤害感受，其中进餐持续时间的延长与TMJ深部伤害感受的强度直接相关。通过定量促炎基因表达来测定炎症。在TMJ CFA注射后，进食时间显著延长，并且在给予生理浓度的雌二醇后，这种延长在去势但不完整的雄性中显著减弱。生理浓度的17β-雌二醇也显著增加了去势雄性动物炎症TMJ中IL-6的表达，而17β-雌二醇没有改变IL-1β、CXCL 2和CCL 20的表达。去势增加促炎介质IL-6、IL-1β和CXCL 2，表明雄性激素具有抗炎作用。三叉神经节CGRP无变化。与雌性大鼠相似，颞下颌关节炎症的雄性大鼠在用生理浓度的雌二醇治疗后表现出降低的伤害性反应，这表明雌二醇治疗的效果不受雄性大鼠组织过程的限制。
Previous studies have shown 17β-estradiol will reduce temporomandibular joint (TMJ) inflammation and hypersensitivity in female rats. Although, male rats contain significant amounts of estradiol it was unknown whether a physiological concentration of 17β-estradiol would attenuate male TMJ inflammation and nociception. Intact and castrated rats were given a physiological concentration of estradiol to examine first, if estradiol will affect male TMJ nociception/inflammation and second, if administration of estradiol would act synergistically with endogenous male hormones to attenuate TMJ nociception. The hormonally treated rats were given TMJ injections of complete Freund’s adjuvant (CFA) and then nociception was measured using a validated method in which a lengthening in meal duration is directly correlated to the intensity of deep TMJ nociception. Inflammation was assayed by quantitating pro-inflammatory gene expression. Meal duration was significantly lengthened after TMJ CFA injection and this lengthening was significantly attenuated in the castrated but not intact males after administering a physiological concentration estradiol. A physiological concentration of 17β-estradiol also significantly increased IL-6 expression in the inflamed TMJ of castrated males while 17β-estradiol did not alter IL-1β, CXCL2 and CCL20 expression. Castration increased pro-inflammatory mediators IL-6, IL-1β and CXCL2 suggesting male sex hormones were anti-inflammatory. CGRP in the trigeminal ganglia was unchanged. Similar to females, male rats with TMJ inflammation showed a reduced nociceptive response after treatment with a physiological concentration of estradiol suggesting the effects of estradiol treatment were not constrained by organizational processes in the males.
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影响因子：
4.4
作者：
Kagari, T;Doi, H;Shimozato, T
通讯作者：
Shimozato, T
影响因子：
1.9
作者：
Alstergren, P;Ernberg, M;Kopp, S
通讯作者：
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影响因子：
3
作者：
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Harper, RP
DOI：
10.1111/j.1440-1681.1995.tb02012.x
发表时间：
1995-05-01
影响因子：
2.9
作者：
ALI, BH;SHARIF, SI;ELKADI, A
通讯作者：
ELKADI, A
影响因子：
7.6
作者：
Goulet, JP;Lavigne, GJ;Lund, JP
通讯作者：
Lund, JP