Cell type-dependent expression of tubulins in Physarum.
Cell type-dependent expression of tubulins in Physarum.
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DOI：
10.1083/jcb.97.6.1852
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发表时间：
1983-12
期刊：
影响因子：
--
通讯作者：
Dove WF
中科院分区：
文献类型：
--
作者：
Burland TG;Gull K;Schedl T;Boston RS;Dove WF
粘液泡菌或疟原虫全细胞裂解产物的双向凝胶电泳法分离了三种α-微管蛋白和两种β-微管蛋白。用于鉴定微管蛋白的标准包括体外自组装成微管的粘阿米巴微管蛋白在二维凝胶上的迁移，葡萄球菌V8蛋白酶和胰凝乳酶的多肽图谱，针对β-微管蛋白的单抗的免疫沉淀，最后通过克隆的微管蛋白DNA序列杂交选择特定的mRNA，然后进行体外翻译。观察到绒毛虫微管蛋白的差异表达。α1和β1微管蛋白在粘阿米巴和疟原虫中均可检测到，α2和β2仅在疟原虫中检测到，α3仅在粘阿米巴相中检测到，可能是鞭毛虫所特有的。在原虫中观察到的微管蛋白种类明显多于粘阿米巴；在原虫中唯一检测到的微管是有丝分裂纺锤体的微管，而粘阿米巴则显示细胞质、中心粒、鞭毛和有丝分裂纺锤体的微管。粘虫和疟原虫RNA的体外翻译表明，α1-微管蛋白、α2-微管蛋白、β1-微管蛋白和β2-微管蛋白有不同的mRNAs，因此可能是不同的基因。因此，粘阿米巴和疟原虫中微管蛋白表达的不同模式反映了微管蛋白基因的差异表达。
Three alpha-tubulins and two beta-tubulins have been resolved by two- dimensional gel electrophoresis of whole cell lysates of Physarum myxamoebae or plasmodia. Criteria used to identify the tubulins included migration on two-dimensional gels with myxamoebal tubulins purified by self-assembly into microtubules in vitro, peptide mapping with Staphylococcus V8 protease and with chymotrypsin, immunoprecipitation with a monoclonal antibody specific for beta- tubulin, and, finally, hybrid selection of specific mRNA by cloned tubulin DNA sequences, followed by translation in vitro. Differential expression of the Physarum tubulins was observed. The alpha 1- and beta 1-tubulins were detected in both myxamoebae and plasmodia; alpha 2 and beta 2 were detected only in plasmodia, alpha 3 was detected only in the myxamoebal phase, and may be specific to the flagellate. Observation of more tubulin species in plasmodia than in myxamoebae was remarkable; the only microtubules detected in plasmodia are those of the mitotoic spindle, whereas myxamoebae display cytoplasmic, centriolar, flagellar, and mitotic-spindle microtubules. In vitro translation of myxamoebal and plasmodial RNAs indicated that there are distinct mRNAs, and therefore probably separate genes, for the alpha 1- , alpha 2-, beta 1-, and beta 2-tubulins. Thus, the different patterns of tubulin expression in myxamoebae and plasmodia reflect differential expression of tubulin genes.
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影响因子：
64.5
作者：
KEMPHUES, KJ;KAUFMAN, TC;RAFF, EC
通讯作者：
RAFF, EC
影响因子：
5.6
作者：
LITTLE, M;LUDUENA, RF;FARRELL, K
通讯作者：
FARRELL, K
影响因子：
7.8
作者：
MCKEITHAN, TW;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL
DOI：
10.1073/pnas.76.8.3991
发表时间：
1979-01-01
影响因子：
11.1
作者：
KEMPHUES, KJ;RAFF, RA;RAFF, EC
通讯作者：
RAFF, EC
影响因子：
64.5
作者：
LEFEBVRE, PA;SILFLOW, CD;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL