Halophilic archaea cultivated from surface sterilized middle-late eocene rock salt are polyploid.
Halophilic archaea cultivated from surface sterilized middle-late eocene rock salt are polyploid.
复制标题
DOI：
10.1371/journal.pone.0110533
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Oksanen HM
中科院分区：
文献类型：
--
作者：
Jaakkola ST;Zerulla K;Guo Q;Liu Y;Ma H;Yang C;Bamford DH;Chen X;Soppa J;Oksanen HM
活细菌和古菌已经从世界各地的几个岩盐矿床中分离出来，这些岩盐矿床的年龄高达数亿年。影响其寿命的一个关键因素是保持其基因组DNA完整的能力，这需要有效的修复机制。已知多倍体微生物对突变和DNA损伤的抵抗力增加，并且有人认为，来自深埋岩盐的微生物将携带其基因组的几个拷贝。在这里，可培养的嗜盐微生物分离自表面灭菌的中晚始新世（38-41万年前）岩盐样品，从800米深的云英盐矿，中国。获得了8个独特的分离物，它们代表两个盐古菌属，盐杆菌属和盐层菌属。我们使用实时PCR显示我们的分离物是多倍体，在指数生长期每个细胞的基因组拷贝数为11-14个基因组。在稳定生长期，倍性水平略微下调，但细胞仍具有6-8的平均基因组拷贝数。生活在古岩盐中的嗜盐古菌的多倍性可能是解释这些生物如何能够克服在埋藏期间延长生存的挑战的一个因素。
Live bacteria and archaea have been isolated from several rock salt deposits of up to hundreds of millions of years of age from all around the world. A key factor affecting their longevity is the ability to keep their genomic DNA intact, for which efficient repair mechanisms are needed. Polyploid microbes are known to have an increased resistance towards mutations and DNA damage, and it has been suggested that microbes from deeply buried rock salt would carry several copies of their genomes. Here, cultivable halophilic microbes were isolated from a surface sterilized middle-late Eocene (38–41 million years ago) rock salt sample, drilled from the depth of 800 m at Yunying salt mine, China. Eight unique isolates were obtained, which represented two haloarchaeal genera, Halobacterium and Halolamina. We used real-time PCR to show that our isolates are polyploid, with genome copy numbers of 11–14 genomes per cell in exponential growth phase. The ploidy level was slightly downregulated in stationary growth phase, but the cells still had an average genome copy number of 6–8. The polyploidy of halophilic archaea living in ancient rock salt might be a factor explaining how these organisms are able to overcome the challenge of prolonged survival during their entombment.
登录
查看更多内容
嗜盐古菌中受调控的多倍体。
影响因子：
3.7
作者：
Breuert S;Allers T;Spohn G;Soppa J
通讯作者：
Soppa J
DOI：
10.1007/s10482-014-0145-0
发表时间：
2014-05-01
影响因子：
2.6
作者：
Cha, In-Tae;Yim, Kyung June;Nam, Young-Do
通讯作者：
Nam, Young-Do
影响因子：
5.1
作者：
Atanasova, Nina S.;Roine, Elina;Oksanen, Hanna M.
通讯作者：
Oksanen, Hanna M.
影响因子：
5.1
作者：
Gramain, Audrey;Chong Diaz, Guillermo;McGenity, Terry J.
通讯作者：
McGenity, Terry J.
影响因子：
3.2
作者：
DALY, MJ;MINTON, KW
通讯作者：
MINTON, KW