Development of carbohydrate based next-generation anti-pertussis vaccines.
Development of carbohydrate based next-generation anti-pertussis vaccines.
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DOI：
10.1016/j.bmc.2022.117066
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发表时间：
2022-11-15
影响因子：
3.5
通讯作者：
Huang, Xuefei
中科院分区：
文献类型：
--
作者：
Wang, Peng;Ramadan, Sherif;Dubey, Purnima;Deora, Rajendar;Huang, Xuefei
百日咳是一种由革兰氏阴性细菌病原体百日咳博德特氏菌引起的高度传染性呼吸道疾病。尽管全球疫苗接种率很高，但百日咳仍在全球范围内卷土重来。在这里，我们讨论了当前百日咳疫苗的开发及其局限性，这凸显了对能够预防该疾病并防止携带者状态发展的新疫苗的需求，从而减少传播。 Bp 的脂寡糖对于疫苗开发来说是一种有吸引力的抗原，因为抗聚糖抗体可能具有杀菌活性。脂寡糖的结构已被确定并分析了其免疫学特性。已经提出了实现表达、分离和生物共轭的策略。然而，大规模获得高纯度的糖仍然是主要障碍之一。化学合成提供了一种补充方法，以纯净且结构明确的形式获取碳水化合物表位。讨论了非还原端百日咳五糖的首次全合成。合成聚糖与强大的免疫原性载体噬菌体Qβ结合，产生高水平且持久的抗聚糖IgG抗体，为开发具有高杀菌活性和生物相容性的新一代抗百日咳疫苗铺平了道路。
Pertussis is a highly contagious respiratory disease caused by the Gram-negative bacterial pathogen, Bordetella pertussis. Despite high global vaccination rates, pertussis is resurging worldwide. Here we discuss the development of current pertussis vaccines and their limitations, which highlight the need for new vaccines that can protect against the disease and prevent development of the carrier state, thereby reducing transmission. The lipo-oligosaccharide of Bp is an attractive antigen for vaccine development as the anti-glycan antibodies could have bactericidal activities. The structure of the lipo-oligosaccharide has been determined and its immunological properties analyzed. Strategies enabling the expression, isolation, and bioconjugation have been presented. However, obtaining the saccharide on a large scale with high purity remains one of the main obstacles. Chemical synthesis provides a complementary approach to accessing the carbohydrate epitopes in a pure and structurally well-defined form. The first total synthesis of the non-reducing end pertussis pentasaccharide is discussed. The conjugate of the synthetic glycan with a powerful immunogenic carrier, bacteriophage Qβ, results in high levels and long-lasting anti-glycan IgG antibodies, paving the way for the development of a new generation of anti-pertussis vaccines with high bactericidal activities and biocompatibilities.
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影响因子：
5.2
作者：
Belcher T;Dubois V;Rivera-Millot A;Locht C;Jacob-Dubuisson F
通讯作者：
Jacob-Dubuisson F
DOI：
10.1099/00221287-137-4-905
发表时间：
1991-04-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
ARCHAMBAULT, D;RONDEAU, P;BRODEUR, BR
通讯作者：
BRODEUR, BR
影响因子：
3.1
作者：
AlBitar-Nehme, Sami;Basheer, Soorej M.;Caroff, Martine
通讯作者：
Caroff, Martine
影响因子：
3.7
作者：
Hoo R;Lam JH;Huot L;Pant A;Li R;Hot D;Alonso S
通讯作者：
Alonso S
核心寡糖的保守结构特征来自Bordetella属的呼吸道病原体的脂多糖，仅通过NMR光谱法分析。
影响因子：
5.6
作者：
Ucieklak K;Koj S;Niedziela T
通讯作者：
Niedziela T