Increased production of IL-4 and IL-12p40 from bronchoalveolar lavage cells are biomarkers of Mycobacterium tuberculosis in the sputum.
Increased production of IL-4 and IL-12p40 from bronchoalveolar lavage cells are biomarkers of Mycobacterium tuberculosis in the sputum.
复制标题
DOI：
10.1371/journal.pone.0059461
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Weiden MD
中科院分区：
文献类型：
--
作者：
Nolan A;Fajardo E;Huie ML;Condos R;Pooran A;Dawson R;Dheda K;Bateman E;Rom WN;Weiden MD
结核病每年在世界范围内造成145万人死亡，其中大部分发生在发展中国家。活动性结核病代表免疫系统无法控制空气传播的潜伏感染。痰涂片上的抗酸杆菌(AFB)是传染性的生物标志物。我们招募了73名广泛浸润性肺结核患者参加一项使用支气管肺泡灌洗(BAL)的研究，以采集肺免疫细胞并测定BAL细胞细胞因子的产生。所有患者都有痰培养显示结核分枝杆菌，59/73(81%)的患者通过痰镜检发现了AFB。与涂片阴性患者相比，初诊时有吸烟史的涂阳患者比例较高，体温为38.50℃，BAL细胞/ml较高，BAL中淋巴细胞百分比较低，BAL细胞培养上清液中IL-4和IL-12p40较高。AFB涂片与其他BAL及血清细胞因子无相关性。IL-4升高与BAL PMN相关，与BAL淋巴细胞呈负相关。在多变量Logistic模型中，BAL IL-4和IL-12p40每增加10倍，AFB涂片阳性的几率分别增加7.4倍和2.2倍。BAL细胞产生的IL-4和IL-12p40的增加是放射性晚期肺结核患者痰中黄曲霉毒素的生物标志物。它们可能反映了对控制结核病的途径的免疫控制效果较差，导致患者的传染性增加。
Tuberculosis (TB) causes 1.45 million deaths annually world wide, the majority of which occur in the developing world. Active TB disease represents immune failure to control latent infection from airborne spread. Acid-fast bacillus (AFB) seen on sputum smear is a biomarker for contagiousness. We enrolled 73 tuberculosis patients with extensive infiltrates into a research study using bronchoalveolar lavage (BAL) to sample lung immune cells and assay BAL cell cytokine production. All patients had sputum culture demonstrating Mycobacterium tuberculosis and 59/73 (81%) had AFB identified by microscopy of the sputum. Compared with smear negative patients, smear positive patients at presentation had a higher proportion with smoking history, a higher proportion with temperature >38.50 C, higher BAL cells/ml, lower percent lymphocytes in BAL, higher IL-4 and IL-12p40 in BAL cell supernatants. There was no correlation between AFB smear and other BAL or serum cytokines. Increasing IL-4 was associated with BAL PMN and negatively associated with BAL lymphocytes. Each 10-fold increase in BAL IL-4 and IL-12p40 increased the odds of AFB smear positivity by 7.4 and 2.2-fold, respectively, in a multi-variable logistic model. Increasing IL-4 and IL-12p40 production by BAL cells are biomarkers for AFB in sputum of patients who present with radiographically advanced TB. They likely reflect less effective immune control of pathways for controlling TB, leading to patients with increased infectiousness.
登录
查看更多内容
影响因子：
6.4
作者：
Fletcher, HA;Owiafe, P;Brookes, RH
通讯作者：
Brookes, RH
影响因子：
24.3
作者：
Sable, S. B.;Goyal, D.;Khuller, G. K.
通讯作者：
Khuller, G. K.
影响因子：
4.4
作者：
Russell, TD;Yan, QY;Walter, MJ
通讯作者：
Walter, MJ
DOI：
10.1084/jem.20052545
发表时间：
2006-07-10
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Khader SA;Partida-Sanchez S;Bell G;Jelley-Gibbs DM;Swain S;Pearl JE;Ghilardi N;Desauvage FJ;Lund FE;Cooper AM
通讯作者：
Cooper AM
DOI：
10.1126/science.1209985
发表时间：
2011-10-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rosas-Ballina M;Olofsson PS;Ochani M;Valdés-Ferrer SI;Levine YA;Reardon C;Tusche MW;Pavlov VA;Andersson U;Chavan S;Mak TW;Tracey KJ
通讯作者：
Tracey KJ