Qualitative and Quantitative Analysis of Phenolic Acids, Flavonoids and Iridoid Glycosides in Yinhua Kanggan Tablet by UPLC-QqQ-MS/MS.
Qualitative and Quantitative Analysis of Phenolic Acids, Flavonoids and Iridoid Glycosides in Yinhua Kanggan Tablet by UPLC-QqQ-MS/MS.
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DOI：
10.3390/molecules200712209
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发表时间：
2015-07-03
期刊：
影响因子：
--
通讯作者：
Chu K
中科院分区：
文献类型：
--
作者：
Lin Y;Xu W;Huang M;Xu W;Li H;Ye M;Zhang X;Chu K
建立了一种简单、快速、特异的超高效液相色谱-三重四极杆质谱方法，用于分析治疗上呼吸道感染、发烧、咳嗽和咽痛的中药银花抗感片（YHKGT）中的 29 种生物活性成分（10 种酚酸、16 种黄酮类化合物和 3 种环烯醚萜苷）。使用 Waters Cortecs UPLC C18 色谱柱 (50 × 2.1 mm, 1.6 μm) 并使用水-0.1% 甲酸和乙腈进行梯度洗脱，成功实现了分离。采用极性切换模式优化多反应监测条件。分析方法的线性度、精密度和准确度均经过验证。 29 种标记化合物的校准曲线显示出良好的线性回归 (r > 0.9982)。 29 种分析物的检测限 (LOD) 和定量限 (LOQ) 分别在 0.03–4.99 ng/mL 和 0.16–14.87 ng/mL 范围内。日内精密度、日间精密度、重复性和稳定性的相对标准偏差（RSD）值分别小于2.79%、4.87%、4.18%和4.71%。 29 种标记化合物的回收率范围为 94.67%–104.78% (RSD ≤ 4.72%)。这些结果表明，所开发的方法对于 YHKGT 的质量评价是有效的。
A simple, rapid and specific ultra-performance liquid chromatography-triple quadrupole mass spectrometry method was developed for the analysis of 29 bioactive components (10 phenolic acids, 16 flavonoids, and three iridoid glycosides) in Yinhua Kanggan tablet (YHKGT), a herbal prescription used for treating upper respiratory infections, fevers, coughs and pharyngalgia. The separation was successfully achieved using a Waters Cortecs UPLC C18 column (50 × 2.1 mm, 1.6 μm) and gradient elution with water-0.1% formic acid and acetonitrile. Polarity switching mode was used in the optimization of multiple reaction monitoring conditions. The analytical method was validated for linearity, precision and accuracy. Calibration curves for the 29 marker compounds showed good linear regression (r > 0.9982). The limits of detection (LOD) and limits of quantification (LOQ) for the 29 analytes were in the range of 0.03–4.99 ng/mL and 0.16–14.87 ng/mL, respectively. The relative standard deviation (RSD) values of intra-day precision, inter-day precision, repeatability, and stability were less than 2.79%, 4.87%, 4.18% and 4.71%, respectively. The recoveries of the 29 marker compounds were in the range of 94.67%–104.78% (RSD ≤ 4.72%). These results have shown that this developed method was efficient for the quality evaluation of YHKGT.
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发表时间：
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影响因子：
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