Serum GP73 is complementary to AFP and GGT-II for the diagnosis of hepatocellular carcinoma.
Serum GP73 is complementary to AFP and GGT-II for the diagnosis of hepatocellular carcinoma.
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DOI：
10.3892/ol.2013.1522
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发表时间：
2013-10
期刊：
影响因子：
2.9
通讯作者：
Chen BY
中科院分区：
文献类型：
--
作者：
Hou SC;Xiao MB;Ni RZ;Ni WK;Jiang F;Li XY;Lu CH;Chen BY
高尔基蛋白73 （GP73）是一种常驻的高尔基II型跨膜蛋白，据报道在慢性肝病，特别是肝细胞癌（HCC）中显著增加。然而，血清GP73是否为HCC诊断的可靠血清标志物尚不清楚。本研究的目的是评价血清GP73对HCC患者的诊断价值，并确定血清GP73联合α-胎蛋白（AFP）和γ-谷氨酰转移酶同工酶II （GGT-II）对HCC的诊断准确性。采用时间分辨荧光免疫法（TRFIA）和酶联免疫吸附法（ELISA）检测79例HCC患者血清GP73水平，其中肝硬化16例，慢性肝炎30例，健康人28例。分析血清GP73与肿瘤大小、HCC分级的相关性，评价血清GP73与AFP、GGT-II的互补诊断价值。建立了检测血清GP73的TRFIA方法，具有灵敏度高、重复性好等优点。HCC患者血清GP73表达水平明显高于肝硬化、慢性肝炎及健康人。根据受试者工作特征（ROC）曲线，截断值为78.1 ng/l的HCC诊断敏感性和特异性分别为73.4和79.0%。血清GP73与肿瘤大小、分级无相关性，血清GP73与AFP、GGT-II无相关性。TRFIA检测GP73、AFP和GGT-II对HCC的诊断敏感性分别为73.4、55.6和68.4%，特异性分别为80.0、86.7和97.1%。这些指标的联合测定将HCC的诊断敏感性提高到96.3%。TRFIA是一种灵敏、可重复的血清GP73检测方法。肝细胞癌组血清GP73水平明显高于良性肝病组。血清GP73可作为HCC的潜在独立诊断候选者，联合检测血清GP73、AFP和GGT-II可提高HCC的诊断效率。
Golgi protein 73 (GP73) is a resident Golgi type II transmembrane protein that has been reported to markedly increase in chronic liver disease, particularly in hepatocellular carcinoma (HCC). However, it remains unclear as to whether serum GP73 represents a reliable serum marker for the diagnosis of HCC. The aim of the present study was to evaluate the diagnostic value of serum GP73 in patients with HCC and to determine the diagnostic accuracy of measuring serum GP73 in combination with α-fetoprotein (AFP) and γ-glutamyl transferase isoenzyme II (GGT-II) in HCC. Serum GP73 was detected using a time-resolved fluorescence immunological assay (TRFIA) and enzyme-linked immunosorbent assay (ELISA) in 79 HCC cases, including 16 liver cirrhosis, 30 chronic hepatitis and 28 healthy individuals. The correlation between serum GP73 and tumor size and HCC grading was analyzed and the complementary diagnostic value of serum GP73, AFP and GGT-II was evaluated. TRFIA was established for the detection of serum GP73 and was sensitive and reproducible. The expression levels of serum GP73 were markedly higher in the patients with HCC when compared with those of the individuals with liver cirrhosis and chronic hepatitis or the healthy individuals. According to the receiver operating characteristic (ROC) curve, diagnostic sensitivity and specificity for HCC with a cut-off value of 78.1 ng/l were 73.4 and 79.0%, respectively. However, no correlation was identified among serum GP73 and tumor size or grading, and no correlations were identified among serum GP73, AFP and GGT-II. The diagnostic sensitivities for HCC, as detected by TRFIA of GP73, AFP and GGT-II, were 73.4, 55.6 and 68.4%, respectively, and the specificities were 80.0, 86.7 and 97.1%, respectively. The combined determination of these markers increased the diagnostic sensitivity to 96.3% for HCC. TRFIA functions as a sensitive and replicable assay for the detection of serum GP73. The levels of serum GP73 were significantly higher in the HCC group when compared with the individuals with benign liver diseases. Serum GP73 may serve as a potential independent diagnostic candidate for HCC and the combined determination of serum GP73, AFP and GGT-II may increase the diagnostic efficiency of HCC.
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影响因子：
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作者：
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