Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue.
Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue.
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DOI：
10.1038/s41551-021-00769-y
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发表时间：
2021-10
影响因子：
28.1
通讯作者：
中科院分区：
文献类型：
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虽然通过粘合剂密封受损组织具有优于缝合或钉合的潜在优点，但现有的组织粘合剂不能在覆盖有体液（例如血液）的组织上形成快速或牢固的粘附。相比之下，藤壶的胶水，由嵌入在富含脂质的基质中的粘附蛋白组成，可以牢固地粘附在潮湿和污染的表面上。在这里，我们报告了一种藤壶启发的糊剂，能够形成快速和强大的止血密封的各种组织。该糊剂由疏水性油基质和生物粘附性微粒组成，以实现藤壶激发的机制，从而通过疏水性基质排斥血液。随后，生物粘附性微粒在温和的压力下与下层组织交联。藤壶灵感糊剂可在各种组织环境中提供快速（小于15 s）、坚韧（界面韧性超过300 J m−2）、坚固（剪切和拉伸强度超过70 kPa，爆破压力超过350 mmHg）和不依赖血液凝固的止血密封。我们在啮齿动物模型中验证了藤壶灵感糊剂的体外和体内生物相容性和生物降解性。与市售止血剂相比，我们进一步证明了藤壶灵感糊剂在离体猪主动脉、体内大鼠肝脏和心脏以及体内猪肝模型中用于快速止血密封的潜在应用。
Whilst sealing damaged tissues by adhesives has potential advantages over suturing or stapling, existing tissue adhesives cannot form rapid or robust adhesion on tissues covered with body fluids such as blood. In contrast, the glues of barnacles, consisting of adhesive proteins embedded in a lipid-rich matrix, can strongly adhere to wet and contaminated surfaces. Here we report a barnacle-inspired paste capable of forming rapid and robust hemostatic sealing of diverse tissues. The paste is composed of a hydrophobic oil matrix and bioadhesive microparticles to implement the barnacle-inspired mechanism to repel blood through the hydrophobic matrix. Subsequently, the bioadhesive microparticles crosslink with underlying tissues under gentle pressure. The barnacle-inspired paste can provide rapid (less than 15 s), tough (interfacial toughness over 300 J m−2), strong (shear and tensile strength over 70 kPa, burst pressure over 350 mmHg), and blood coagulation-independent hemostatic sealing in a variety of tissue environments. We validate in vitro and in vivo biocompatibility and biodegradability of the barnacle-inspired paste in rodent models. We further demonstrate potential applications of the barnacle-inspired paste for rapid hemostatic sealing in ex vivo porcine aorta, in vivo rat liver and heart, and in vivo porcine liver models in comparison with commercially-available hemostatic agents.
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DOI：
10.1016/j.ejpb.2015.05.022
发表时间：
2015-09
期刊：
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
影响因子：
--
作者：
Annabi N;Yue K;Tamayol A;Khademhosseini A
通讯作者：
Khademhosseini A
DOI：
10.1016/j.ijadhadh.2006.03.004
发表时间：
2007-03-01
影响因子：
3.4
作者：
Khandeparker, Lidita;Anil, Arga Chandrashekhar
通讯作者：
Anil, Arga Chandrashekhar
影响因子：
16.6
作者：
Hong, Yi;Zhou, Feifei;Ouyang, Hongwei
通讯作者：
Ouyang, Hongwei
影响因子：
2.1
作者：
Corral, Mitra;Ferko, Nicole;Chang, Eunice
通讯作者：
Chang, Eunice
影响因子：
2.8
作者：
Essock-Burns, Tara;Gohad, Neeraj V.;Rittschof, Daniel
通讯作者：
Rittschof, Daniel