Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation
Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation
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CP190 的 BTB 结构域与 Su(Hw) 中两个相邻区域之间的相互作用是绝缘体复合物形成所必需的
DOI：
10.1007/s00412-017-0645-6
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发表时间：
2017
期刊：
影响因子：
1.6
通讯作者：
A. Golovnin
中科院分区：
文献类型：
--
作者：
L. Melnikova;M. Kostyuchenko;V. Molodina;Alexander Parshikov;P. Georgiev;A. Golovnin
果蝇绝缘子复合体研究最多的是两种含BTB的蛋白质，Mod（mdg 4）-67.2亚型和CP 190，它们通过与DNA结合结构蛋白Su（Hw）相互作用协同募集到染色质中。虽然Mod（mdg 4）-67.2仅与Su（Hw）相互作用，但CP 190与许多其他结构蛋白相互作用。尽管CP 190对Su（Hw）绝缘体的活性至关重要，但这些蛋白质之间的相互作用尚未研究。因此，我们已经进行了详细的分析，在苏（Hw）和CP 190之间的相互作用所涉及的领域。结果表明，CP 190的BTB结构域与Su（Hw）N端的两个相邻区域相互作用。在Mod（mdg 4）-67.2的存在下，Su（Hw）中任一区域的缺失仅微弱地影响CP 190向Su（Hw）位点的募集。Su（Hw）中两个区域的缺失阻止了其与CP 190的相互作用。使用体内突变，我们发现与Su（Hw）和Mod（mdg 4）-67.2的相互作用对于将CP 190募集到Su（Hw）基因组位点至关重要。
The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many other architectural proteins. In spite of the fact that CP190 is critical for the activity of Su(Hw) insulators, interaction between these proteins has not been studied yet. Therefore, we have performed a detailed analysis of domains involved in the interaction between the Su(Hw) and CP190. The results show that the BTB domain of CP190 interacts with two adjacent regions at the N-terminus of Su(Hw). Deletion of either region in Su(Hw) only weakly affected recruiting of CP190 to the Su(Hw) sites in the presence of Mod(mdg4)-67.2. Deletion of both regions in Su(Hw) prevents its interaction with CP190. Using mutations in vivo, we found that interactions with Su(Hw) and Mod(mdg4)-67.2 are essential for recruiting of CP190 to the Su(Hw) genomic sites.
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