Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin.
Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin.
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DOI：
10.1101/gr.174169.114
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发表时间：
2015-01
期刊：
影响因子：
7
通讯作者：
Georgiev P
中科院分区：
文献类型：
--
作者：
Maksimenko O;Bartkuhn M;Stakhov V;Herold M;Zolotarev N;Jox T;Buxa MK;Kirsch R;Bonchuk A;Fedotova A;Kyrchanova O;Renkawitz R;Georgiev P
绝缘子是调节核结构的多蛋白-DNA复合物。果蝇CP 190蛋白是DNA结合绝缘子蛋白Su（Hw）、CTCF和BEAF-32的辅因子。事实上，CP 190已被发现在基因组位点缺乏任何一个已知的绝缘子因子至今尚未解释。我们已经确定了两个DNA结合锌指蛋白，皮塔，和一个新的因子命名为ZIPIC，在体内和体外与CP 190在特定的相互作用域。这些蛋白质的基因组结合位点与CP 190以及CTCF和BEAF-32成簇。Pita或ZIPIC的模型结合位点表现出部分增强子阻断活性，并保护基因表达免受PRE介导的沉默。CTCF结合的MCP绝缘子序列的功能需要Pita的结合。这些结果确定了两个新的绝缘子蛋白，并强调了CP 190的统一功能，它可以被许多DNA结合绝缘子蛋白招募。
Insulators are multiprotein–DNA complexes that regulate the nuclear architecture. The Drosophila CP190 protein is a cofactor for the DNA-binding insulator proteins Su(Hw), CTCF, and BEAF-32. The fact that CP190 has been found at genomic sites devoid of either of the known insulator factors has until now been unexplained. We have identified two DNA-binding zinc-finger proteins, Pita, and a new factor named ZIPIC, that interact with CP190 in vivo and in vitro at specific interaction domains. Genomic binding sites for these proteins are clustered with CP190 as well as with CTCF and BEAF-32. Model binding sites for Pita or ZIPIC demonstrate a partial enhancer-blocking activity and protect gene expression from PRE-mediated silencing. The function of the CTCF-bound MCP insulator sequence requires binding of Pita. These results identify two new insulator proteins and emphasize the unifying function of CP190, which can be recruited by many DNA-binding insulator proteins.
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