Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis.
Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis.
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miR-9作为鼻咽癌转移潜在生物标志物的预测价值。
DOI：
10.1038/bjc.2013.751
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发表时间：
2014-01-21
影响因子：
8.8
通讯作者：
Li, X.
中科院分区：
文献类型：
--
作者：
Lu, J.;Xu, X.;Liu, X.;Peng, Y.;Zhang, B.;Wang, L.;Luo, H.;Peng, X.;Li, G.;Tian, W.;He, M.;Li, X.
鼻咽癌（Nasopharyngealcarcinoma，NPC）具有独特的地理分布和转移倾向。本研究旨在检测鼻咽癌患者血浆中microRNA（miRNA）的表达谱，探讨其在疾病发生、发展中的临床意义。本研究分为四个步骤：（1）使用微阵列分析和定量PCR验证来确认差异表达的miRNA;（2）比较NPC进展过程中血浆miR-9水平;（3）评估血浆miR-9作为NPC转移生物标志物的预测性能;以及（4）比较治疗前和治疗后样本之间的血浆miR-9水平。血浆微阵列分析发现33个差异表达的miRNA在NPC患者和健康志愿者之间。通过两阶段验证证实了鼻咽癌患者中miR-9水平的显著下降。血浆miR-9水平低与淋巴结浸润程度差、TNM分期高相关。血浆miR-9检测对鉴别鼻咽癌局部和转移具有较高的敏感性和特异性。此外，与治疗前样本相比，治疗后血浆中的血浆miR-9水平显着升高。我们的研究报告，血浆miR-9可能作为一个有用的生物标志物，预测NPC转移和监测肿瘤动力学。
Nasopharyngeal carcinoma (NPC) has a distinctive geographic distribution and is characterised by its strong tendency of metastasis. We aimed to examine the microRNA (miRNA) expression profiles in plasma samples of NPC patients to explore their clinical significance in disease development and progression. This study was divided into four steps: (1) confirmation of differentially expressed miRNAs using microarray analysis and quantitative PCR validation; (2) comparison of plasma miR-9 levels during NPC progression; (3) evaluation of the predictive performance of plasma miR-9 as a biomarker for NPC metastasis; and (4) comparison of plasma miR-9 levels between pre- and post-treatment samples. Plasma microarray profiling identified 33 differentially expressed miRNAs between NPC patients and healthy volunteers. The significantly declined level of miR-9 in NPC patients was confirmed through two-stage validation. The low level of plasma miR-9 was significantly correlated with worse lymphatic invasion and advanced TNM stage. The plasma miR-9 could distinguish locoregional from metastatic NPC cases with a high sensitivity and specificity. Furthermore, the plasma miR-9 level was significantly elevated in post-treatment plasma compared with those pre-treatment samples. Our study reports that plasma miR-9 may serve as a useful biomarker to predict NPC metastasis and to monitor tumour dynamics.
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DOI：
10.1111/j.1750-3639.2008.00184.x
发表时间：
2009-07
期刊：
Brain pathology (Zurich, Switzerland)
影响因子：
--
作者：
Nass D;Rosenwald S;Meiri E;Gilad S;Tabibian-Keissar H;Schlosberg A;Kuker H;Sion-Vardy N;Tobar A;Kharenko O;Sitbon E;Lithwick Yanai G;Elyakim E;Cholakh H;Gibori H;Spector Y;Bentwich Z;Barshack I;Rosenfeld N
通讯作者：
Rosenfeld N
影响因子：
6
作者：
Nie, Kui;Gomez, Mario;Tam, Wayne
通讯作者：
Tam, Wayne
影响因子：
8
作者：
Hildebrandt, M. A. T.;Gu, J.;Wu, X.
通讯作者：
Wu, X.
影响因子：
3.7
作者：
Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.
影响因子：
21.3
作者：
Ma, Li;Young, Jennifer;Prabhala, Harsha;Pan, Elizabeth;Mestdagh, Pieter;Muth, Daniel;Teruya-Feldstein, Julie;Reinhardt, Ferenc;Onder, Tamer T.;Valastyan, Scott;Westermann, Frank;Speleman, Frank;Vandesompele, Jo;Weinberg, Robert A.
通讯作者：
Weinberg, Robert A.