The CIAMIB: a Large and Metabolically Diverse Collection of Inflammation-Associated Bacteria from the Murine Gut.
The CIAMIB: a Large and Metabolically Diverse Collection of Inflammation-Associated Bacteria from the Murine Gut.
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肠道炎症直接影响肠道微生物的生长和稳定性，并可能导致微生物群组成的长期变化，从而延长或加剧疾病状态。虽然小鼠模型被广泛用于研究微生物和炎症状态之间的相互作用，但培养的小鼠肠道微生物的缺乏阻碍了确定因果关系的努力。为了解决这个问题，我们正在收集炎症相关小鼠肠道细菌（CIAMIB）。该集合的初始版本包括39个独特细菌物种的41个分离株，覆盖4门，并包含10个以前未培养的分离株，包括1个新的科和7个新的属。该集合显著扩大了可用的Muribaculaceae，Lachnospiraceae和Coriobacteriaceae分离株的数量，并包括来自与炎症相关的属的微生物，如Prevotella和Klebsiella。我们表征了CIAMIB分离株在各种营养条件下的生长，并基于这些分离株的基因组预测了它们的代谢潜力和厌氧发酵能力。我们还提供了第一个代谢分析属Adlercreutzia内的物种，揭示这些代表是硝酸盐还原和严重限制他们的能力，碳水化合物的增长。CIAMIB分离株已完全测序，可作为科学界研究宿主-微生物群相互作用的有力工具。
Gut inflammation directly impacts the growth and stability of commensal gut microbes and can lead to long-lasting changes in microbiota composition that can prolong or exacerbate disease states. While mouse models are used extensively to investigate the interplay between microbes and the inflamed state, the paucity of cultured mouse gut microbes has hindered efforts to determine causal relationships. To address this issue, we are assembling the Collection of Inflammation-Associated Mouse Intestinal Bacteria (CIAMIB). The initial release of this collection comprises 41 isolates of 39 unique bacterial species, covering 4 phyla and containing 10 previously uncultivated isolates, including 1 novel family and 7 novel genera. The collection significantly expands the number of available Muribaculaceae, Lachnospiraceae, and Coriobacteriaceae isolates and includes microbes from genera associated with inflammation, such as Prevotella and Klebsiella. We characterized the growth of CIAMIB isolates across a diverse range of nutritional conditions and predicted their metabolic potential and anaerobic fermentation capacity based on the genomes of these isolates. We also provide the first metabolic analysis of species within the genus Adlercreutzia, revealing these representatives to be nitrate-reducing and severely restricted in their ability to grow on carbohydrates. CIAMIB isolates are fully sequenced and available to the scientific community as a powerful tool to study host-microbiota interactions.
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影响因子：
--
作者：
Auch AF;von Jan M;Klenk HP;Göker M
通讯作者：
Göker M
影响因子：
3.7
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
通讯作者：
Xiang C
影响因子：
5.2
作者：
Gänzle MG;Follador R
通讯作者：
Follador R
DOI：
10.1007/s00018-017-2693-8
发表时间：
2018-01
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Hugenholtz F;de Vos WM
通讯作者：
de Vos WM
影响因子：
4.8
作者：
Choo JM;Kanno T;Zain NM;Leong LE;Abell GC;Keeble JE;Bruce KD;Mason AJ;Rogers GB
通讯作者：
Rogers GB