Identification of a specific domain of Porphyromonas gingivalis Hgp44 responsible for adhesion to Treponema denticola
Identification of a specific domain of Porphyromonas gingivalis Hgp44 responsible for adhesion to Treponema denticola
复制标题
牙龈卟啉单胞菌 Hgp44 负责粘附齿垢密螺旋体的特定结构域的鉴定
DOI：
10.1093/femspd/fty047
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Ishihara Kazuyuki
中科院分区：
文献类型：
--
作者：
Yoshikawa Kouki;Kikuchi Yuichiro;Kokubu Eitoyo;Imamura Kentaro;Saito Atsushi;Ishihara Kazuyuki
牙周两种病原菌牙龈卟啉单胞菌和齿密螺旋体之间的相互作用有助于菌斑生物膜的形成。齿状病毒通过其黏附/血凝素结构域(Hgp44)。在这项研究中，我们研究了特定的结构域OFP。牙周炎Hgp44负责粘连TOT。含齿状病毒的表达载体p.牙周病毒Hgp44DNA序列编码1-419个氨基酸残基。通过聚合酶链式反应扩增和自连，获得了重组蛋白r-Hgp44(残基1-419)、r-Hgp441(残基1-124)、r-Hgp442(1-199)、r-Hgp443(1-316)、r-Hgp444(199-419)、r-Hgp445(124-198)和r-Hgp446(199-316)，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹证实。R-Hgp44、r-Hgp443和r-Hgp446的粘附性较强。用酶联免疫吸附试验测定，比对照有更高的滴度。R-Hgp446降低了共聚集的OFP。牙周炎和牙周炎。丹蒂科拉。扫描电子显微镜和激光共聚焦扫描显微镜分析表明，r-Hgp446减少了双物种生物膜的形成。我们的结果表明，残基199-316个OFP。牙周炎Hgp44主要负责TOT的粘连。抑制这一结构域可能会破坏牙周病理性生物膜的形成和成熟。
Interaction between two periodontal pathogens,Porphyromonas gingivalisandTreponema denticola, contributes to plaque biofilm formation.Porphyromonas gingivalisforms aggregates withT. denticolathrough its adhesion/hemagglutinin domain (Hgp44). In this study, we investigated the specific domain ofP. gingivalisHgp44 responsible for adhesion toT. denticolausing expression vectors harboringP. gingivalisHgp44 DNA sequences encoding amino acid residues 1–419. Six plasmids harboring fragments in this region were generated by PCR amplification and self-ligation, and recombinant proteins r-Hgp44 (residues 1–419), r-Hgp441(residues 1–124), r-Hgp442(1–199), r-Hgp443(1–316), r-Hgp444(199–419), r-Hgp445(124–198) and r-Hgp446(199–316) were produced, as confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting. r-Hgp44, r-Hgp443and r-Hgp446showed greater adhesion toT. denticolasonicates than the control, as determined by enzyme-linked immunosorbent assay. r-Hgp446reduced the coaggregation ofP. gingivalisandT. denticola. Scanning electron and confocal laser scanning microscopy analyses revealed that r-Hgp446reduced dual-species biofilm formation. Our results indicate that residues 199–316 ofP. gingivalisHgp44 are mainly responsible for adhesion toT. denticola; inhibiting this domain could potentially disrupt periodontopathic biofilm formation and maturation.
登录
查看更多内容
影响因子：
2.6
作者：
Kamaguchi, A;Nakayama, K;Baba, H
通讯作者：
Baba, H
影响因子：
2.1
作者：
M. Yamada;A. Ikegami;H. Kuramitsu
通讯作者：
M. Yamada;A. Ikegami;H. Kuramitsu
牙龈拟杆菌培养上清血凝素的纯化及性质
影响因子：
3.1
作者：
K. Okuda;A. Yamamoto;Y. Naito;I. Takazoe;J. Slots;R. Genco
通讯作者：
R. Genco
影响因子：
13.6
作者：
Lamont RJ;Hajishengallis G
通讯作者：
Hajishengallis G
由牙龈卟啉单胞菌的牙龈蛋白酶基因编码的粘附素
DOI：
--
发表时间：
2003
期刊：
Microbiology 149
影响因子：
--
作者：
Kamaguchi A.et al.
通讯作者：
Kamaguchi A.et al.