Novosphingobium and its potential role in chronic obstructive pulmonary diseases: insights from microbiome studies.
Novosphingobium and its potential role in chronic obstructive pulmonary diseases: insights from microbiome studies.
复制标题
新鞘氨醇及其在慢性阻塞性肺疾病中的潜在作用：来自微生物组研究的见解。
DOI：
10.1371/journal.pone.0111150
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Harris JK
中科院分区：
文献类型：
--
作者：
Rutebemberwa A;Stevens MJ;Perez MJ;Smith LP;Sanders L;Cosgrove G;Robertson CE;Tuder RM;Harris JK
肺气道的细菌感染是慢性阻塞性肺病的一些主要并发症的基础，显着影响疾病的进展和结果。细菌的定植可能会进一步协同、放大或触发由香烟烟雾引发的组织损伤途径，从而导致慢性阻塞性肺病的特征性气道炎症和肺泡破坏。我们试图阐明慢性阻塞性肺病不同阶段肺部细菌群的存在和类型，旨在揭示对该疾病病理学的重要见解。对 55 个特征明确的临床肺部样本中的细菌小亚基核糖体 RNA 基因进行测序，揭示了新鞘氨醇菌属的存在。 （>2% 丰度） GOLD 3-GOLD 4 COPD、囊性纤维化患者和一部分对照个体的肺部。 Novosphingobium 特异性定量 PCR 与序列数据和高水平的 Novosphingobium spp 一致。在晚期 COPD 中可以量化，但在其他疾病阶段则无法量化。使用由于吸入香烟烟雾而导致亚急性肺损伤的小鼠模型，与对照小鼠相比，经气管内用帕尼帕滕氏球菌攻击的小鼠的支气管肺泡灌洗中的中性粒细胞和巨噬细胞计数显着较高（p<0.01）。与对照组相比，在室内空气中用帕尼帕特奈氏球菌攻击的小鼠肺组织中中性粒细胞和巨噬细胞的频率增加。然而，我们没有观察到 N. panipatense 与小鼠亚急性香烟烟雾暴露之间的相互作用。总之，Novosphingobium spp。存在于更严重的慢性阻塞性肺病中，并会增加暴露于烟雾的小鼠模型中的炎症。
Bacterial infection of lung airways underlies some of the main complications of COPD, significantly impacting disease progression and outcome. Colonization by bacteria may further synergize, amplify, or trigger pathways of tissue damage started by cigarette smoke, contributing to the characteristic airway inflammation and alveolar destruction of COPD. We sought to elucidate the presence and types of lung bacterial populations in different stages of COPD, aimed at revealing important insights into the pathobiology of the disease. Sequencing of the bacterial small subunit ribosomal RNA gene in 55 well-characterized clinical lung samples, revealed the presence of Novosphingobium spp. (>2% abundance) in lungs of patients with GOLD 3-GOLD 4 COPD, cystic fibrosis and a subset of control individuals. Novosphingobium-specific quantitative PCR was concordant with the sequence data and high levels of Novosphingobium spp. were quantifiable in advanced COPD, but not from other disease stages. Using a mouse model of subacute lung injury due to inhalation of cigarette smoke, bronchoalveolar lavage neutrophil and macrophage counts were significantly higher in mice challenged intratracheally with N. panipatense compared to control mice (p<0.01). Frequencies of neutrophils and macrophages in lung tissue were increased in mice challenged with N. panipatense at room air compared to controls. However, we did not observe an interaction between N. panipatense and subacute cigarette smoke exposure in the mouse. In conclusion, Novosphingobium spp. are present in more severe COPD disease, and increase inflammation in a mouse model of smoke exposure.
登录
查看更多内容
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
DOI：
10.1164/rccm.201210-1913oc
发表时间：
2013-05-15
影响因子：
24.7
作者：
Morris, Alison;Beck, James M.;Weinstock, George M.
通讯作者：
Weinstock, George M.
DOI：
10.1513/pats.200707-088et
发表时间：
2008-05-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
MacIntyre, Neil;Huang, Yuh Chin
通讯作者：
Huang, Yuh Chin
DOI：
10.1513/pats.200707-097th
发表时间：
2007-12-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Martinez, Fernando J
通讯作者：
Martinez, Fernando J