High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain.
High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain.
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DOI：
10.1126/sciadv.abi5464
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发表时间：
2021-12-10
期刊：
影响因子：
13.6
通讯作者：
Razansky D
中科院分区：
文献类型：
--
作者：
Estrada H;Robin J;Özbek A;Chen Z;Marowsky A;Zhou Q;Beck D;le Roy B;Arand M;Shoham S;Razansky D
通过荧光引导经颅超声实现临床前聚焦超声的精确定位和监测。了解经颅超声对啮齿动物大脑的生理影响可能为人体规模磁共振引导聚焦超声方法提供重要的临床前模型。然而，目前缺乏用于高分辨率经颅超声靶向和实时体内跟踪其在小鼠大脑尺度上的影响的精密工具。我们报告了一种多功能双向混合荧光超声 (FLUS) 系统，结合了 0.35 毫米精密球形相控阵超声发射和基于纤维镜的宽视场荧光成像。我们展示了如何将全皮层功能成像和靶向超声传递之间的结合用于经颅映射由可逆热过程引起的先前未记录的局部荧光事件，并对聚焦超声引起的神经活动进行高速大规模记录。因此，FLUS 自然地利用荧光标签和超声波局部生物效应的广泛工具箱来可视化并因果扰乱活体小鼠大脑中的大量正常和病理生理过程。
Precision targeting and monitoring of preclinical focused ultrasound enabled by fluorescence-guided transcranial ultrasound. Understanding the physiological impact of transcranial ultrasound in rodent brains may offer an important preclinical model for human scale magnetic resonance–guided focused ultrasound methods. However, precision tools for high-resolution transcranial ultrasound targeting and real-time in vivo tracking of its effects at the mouse brain scale are currently lacking. We report a versatile bidirectional hybrid fluorescence-ultrasound (FLUS) system incorporating a 0.35-mm precision spherical-phased array ultrasound emission with a fiberscope-based wide-field fluorescence imaging. We show how the marriage between cortex-wide functional imaging and targeted ultrasound delivery can be used to transcranially map previously undocumented localized fluorescence events caused by reversible thermal processes and perform high-speed large-scale recording of neural activity induced by focused ultrasound. FLUS thus naturally harnesses the extensive toolbox of fluorescent tags and ultrasound’s localized bioeffects toward visualizing and causally perturbing a plethora of normal and pathophysiological processes in the living murine brain.
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影响因子：
3.5
作者：
Larrat, B.;Pernot, M.;Tanter, M.
通讯作者：
Tanter, M.
影响因子：
3.3
作者：
Collins, CM;Smith, MB;Turner, R
通讯作者：
Turner, R
影响因子：
16.2
作者：
Grienberger, Christine;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
影响因子：
4.6
作者：
Lee W;Kim HC;Jung Y;Chung YA;Song IU;Lee JH;Yoo SS
通讯作者：
Yoo SS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y