Laser-induced endothelial damage inhibits endothelium-dependent relaxation in the cerebral microcirculation of the mouse.
Laser-induced endothelial damage inhibits endothelium-dependent relaxation in the cerebral microcirculation of the mouse.
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激光诱导的内皮损伤抑制小鼠大脑微循环的内皮依赖性松弛。
DOI：
10.1161/01.res.60.2.169
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发表时间：
1987
影响因子：
20.1
通讯作者：
Povlishock,JT
中科院分区：
文献类型：
--
作者：
Rosenblum,WI;Nelson,GH;Povlishock,JT
这项研究证明了大脑表面小动脉的内皮依赖性松弛。静脉注射后，使用氦氖激光原位损伤内皮。注射伊文思蓝染料，使床对激光敏感。直径为 18 或 36 微米的区域受到损伤，并且在 1 ml 氯化乙酰胆碱或三乙酸缓激肽（80 微克/ml）持续 60 秒的情况下不再松弛。硝普钠（30 微克/毫升）的扩张不受影响。对硝普钠的正常反应加上电子显微镜检查证实血管平滑肌未受伤。当仅存在轻微的超微结构损伤时，内皮依赖性舒张就会受损。下游和上游距激光束中心 80 微米处的膨胀均受到抑制。这表明内皮损伤在激光撞击部位周围扩散。然而，下游的抑制比上游更明显，这意味着下游响应的一部分依赖于从上游位点释放的物质。迄今为止，很少有研究报道体内内皮依赖性舒张，特别是在微循环中。本研究实现了这一点。此外，与大血管中内皮依赖性舒张的体外观察相反，体内消除微循环中内皮依赖性舒张既不需要去除内皮，也不需要损伤大量内皮细胞。由于现已使用三种不同技术损伤内皮来证明微循环中的内皮依赖性松弛，因此可以合理地得出结论，该现象是真实的。
This study demonstrates endothelium-dependent relaxation in the surface arterioles of the brain. A helium-neon laser was used to injure endothelium in situ following i.v. injection of Evans blue dye, which sensitizes the bed to the laser. Areas 18 or 36 micron in diameter were injured and no longer relaxed to either 1 ml of acetylcholine chloride or bradykinin triacetate, 80 micrograms/ml delivered for 60 seconds. Dilations to sodium nitroprusside (30 micrograms/ml) were unaffected. Normal responses to nitroprusside, plus electron microscopy, established that vascular smooth muscle was uninjured. Endothelium-dependent relaxation was impaired when only minor ultrastructural damage was present. Dilation was inhibited downstream and upstream as far as 80 micron from the center of the laser beam. This suggests a spread of endothelium injury around the site of laser impact. However, inhibition was somewhat more marked downstream than upstream, implying that a portion of the downstream response was dependent on a substance released from an upstream site. To date, very few studies have reported endothelium-dependent relaxation in vivo, especially in the microcirculation. The present study accomplishes this. Moreover, in contrast to in vitro observations of endothelium-dependent relaxation in large vessels, the in vivo elimination of endothelium-dependent relaxation in the microcirculation required neither removal of endothelium nor injury to large numbers of endothelium cells. Since endothelium-dependent relaxation in the microcirculation has now been demonstrated using three different techniques to injure endothelium, it is reasonable to conclude that the phenomenon is real.
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Siegel (S.)——行为科学的参数统计
影响因子：
8.3
作者：
Guitton Henri
通讯作者：
Guitton Henri
小鼠大脑表面微小血管中的红细胞速度和速度脉冲
影响因子：
20.1
作者：
W. Rosenblum
通讯作者：
W. Rosenblum
影响因子：
--
作者：
W. Rosenblum;B. Zweifach
通讯作者：
B. Zweifach
脑激肽释放酶-激肽系统在脑循环调节中可能发挥作用的证据。
影响因子：
20.1
作者：
Kamitani,T;Little,MH;Ellis,EF
通讯作者：
Ellis,EF
DOI：
--
发表时间：
1983
期刊：
General Pharmacology
影响因子：
--
作者：
P. Vanhoutte;J. D. De Mey
通讯作者：
J. D. De Mey