SWR-C and INO80 chromatin remodelers recognize nucleosome-free regions near +1 nucleosomes.
SWR-C and INO80 chromatin remodelers recognize nucleosome-free regions near +1 nucleosomes.
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DOI：
10.1016/j.cell.2013.08.043
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发表时间：
2013-09-12
期刊：
影响因子：
64.5
通讯作者：
Pugh BF
中科院分区：
文献类型：
--
作者：
Yen K;Vinayachandran V;Pugh BF
SWR-C/SWR 1和INO 80是多亚基复合物，催化组蛋白变体H2 A. Z从基因起始处的第一个核小体的沉积和去除。它们如何靶向和接合这些+1核小体尚不清楚。使用ChIP-exo，我们确定了酵母基因组中20个亚基的亚核小体位置。SWR-C的Swc 2亚基结合了相邻无核小体区（NFR）中的一个狭窄区域，在那里它将Swr 1亚基定位在H2 A. Z沉积的NFR近端位点上方+1。基因组结合图谱表明，许多亚基具有相当可塑的组织，允许亚基在两个复合物之间整合。促进H2 A/H2A.Z交换的一个结果是整个核小体的周转增强，从而在基因的起始处产生动态染色质。我们的研究结果提供了关于这两个相反的染色质重塑复合物如何选择性地在几乎所有基因的+1核小体发挥作用的统一概念。
SWR-C/SWR1 and INO80 are multi-subunit complexes that catalyze the deposition and removal of histone-variant H2A.Z from the first nucleosome at the start of genes. How they target and engage these +1 nucleosomes is unclear. Using ChIP-exo, we identified the sub-nucleosomal placement of 20 of their subunits across the yeast genome. The Swc2 subunit of SWR-C bound a narrowly defined region in the adjacent nucleosome-free region (NFR), where it positioned the Swr1 subunit over the NFR-proximal site of H2A.Z deposition at +1. The genomic binding maps suggest that many subunits have a rather plastic organization that allows subunits to integrate between the two complexes. One outcome of promoting H2A/H2A.Z exchange was an enhanced turnover of entire nucleosomes, thereby creating dynamic chromatin at the start of genes. Our findings provide unifying concepts on how these two opposing chromatin remodeling complex function selectively at the +1 nucleosome of nearly all genes.
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影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
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Hughes, Timothy R.
影响因子：
64.5
作者：
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通讯作者：
Madhani HD
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14.9
作者：
Gerhold CB;Winkler DD;Lakomek K;Seifert FU;Fenn S;Kessler B;Witte G;Luger K;Hopfner KP
通讯作者：
Hopfner KP
影响因子：
4.8
作者：
Eisen, A;Utley, RT;Côté, J
通讯作者：
Côté, J
影响因子：
4.8
作者：
Jin, JY;Cai, Y;Conaway, JW
通讯作者：
Conaway, JW