The Effect of Single Pyramidal Neuron Firing Within Layer 2/3 and Layer 4 in Mouse V1.
The Effect of Single Pyramidal Neuron Firing Within Layer 2/3 and Layer 4 in Mouse V1.
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DOI：
10.3389/fncir.2018.00029
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发表时间：
2018
影响因子：
3.5
通讯作者：
Smirnakis SM
中科院分区：
文献类型：
--
作者：
Meyer JF;Golshani P;Smirnakis SM
The influence of cortical cell spiking activity on nearby cells has been studied extensively in vitro. Less is known, however, about the impact of single cell firing on local cortical networks in vivo. In a pioneering study, Kwan and Dan (Kwan and Dan,) reported that in mouse layer 2/3 (L2/3), under anesthesia, stimulating a single pyramidal cell recruits ~2.1% of neighboring units. Here we employ two-photon calcium imaging in layer 2/3 of mouse V1, in conjunction with single-cell patch clamp stimulation in layer 2/3 or layer 4, to probe, in both the awake and lightly anesthetized states, how (i) activating single L2/3 pyramidal neurons recruits neighboring units within L2/3 and from layer 4 (L4) to L2/3, and whether (ii) activating single pyramidal neurons changes population activity in local circuit. To do this, it was essential to develop an algorithm capable of quantifying how sensitive the calcium signal is at detecting effectively recruited units (“followers”). This algorithm allowed us to estimate the chance of detecting a follower as a function of the probability that an epoch of stimulation elicits one extra action potential (AP) in the follower cell. Using this approach, we found only a small fraction (<0.75%) of L2/3 cells to be significantly activated within a radius of ~200 μm from a stimulated neighboring L2/3 pyramidal cell. This fraction did not change significantly in the awake vs. the lightly anesthetized state, nor when stimulating L2/3 vs. underlying L4 pyramidal neurons. These numbers are in general agreement with, though lower than, the percentage of neighboring cells (2.1% pyramidal cells and interneurons combined) reported by Kwan and Dan to be activated upon stimulating single L2/3 pyramidal neurons under anesthesia (Kwan and Dan,). Interestingly, despite the small number of individual units found to be reliably driven, we did observe a modest but significant elevation in aggregate population responses compared to sham stimulation. This underscores the distributed impact that single cell stimulation has on neighboring microcircuit responses, revealing only a small minority of relatively strongly connected partners. Patch-clamp stimulation in conjunction with 2-photon imaging shows that activating single layer-2/3 or layer-4 pyramidal neurons produces few (<1% of local units) reliable single-cell followers in L2/3 of mouse area V1, either under light anesthesia or in quiet wakefulness: instead, single cell stimulation was found to elevate aggregate population activity in a weak but highly distributed fashion.
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影响因子：
9.2
作者：
Kwan, Alex C.;Dan, Yang
通讯作者：
Dan, Yang
通过三重免疫染色鉴定小鼠视觉皮层中 GABA 能神经元的多种不同亚型。
影响因子：
2.9
作者：
Gonchar, Yuri;Wang, Quanxin;Burkhalter, Andreas
通讯作者：
Burkhalter, Andreas
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.aac9462
发表时间：
2015-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jiang X;Shen S;Cadwell CR;Berens P;Sinz F;Ecker AS;Patel S;Tolias AS
通讯作者：
Tolias AS
影响因子：
25
作者：
Hofer SB;Ko H;Pichler B;Vogelstein J;Ros H;Zeng H;Lein E;Lesica NA;Mrsic-Flogel TD
通讯作者：
Mrsic-Flogel TD