Global transcriptomic response of bovine endometrium to blastocyst stage embryos.
Global transcriptomic response of bovine endometrium to blastocyst stage embryos.
复制标题
牛子宫内膜对囊胚期胚胎的整体转录组反应。
作者：
C. Passaro;D. Tutt;Sandra Bages Arnal;C. Maicas;R. Laguna;A. Gutiérrez;J. Browne;D. Rath;S. Behura;T. Spencer;T. Fair;P. Lonergan
本研究的目的是（i）研究暴露于囊胚诱导的牛子宫内膜外植体的整体转录组的变化，（ii）研究雄性和雌性囊胚是否在体外子宫内膜转录组中引起差异反应，以及（iii）确定牛子宫内膜是否对小鼠胚胎的存在作出反应。在实验1中，从同一子宫的子宫内膜外植体培养6小时，或没有20个体外产生的牛囊胚。在实验2中，将子宫内膜外植体与通过IVF体外产生的雄性或雌性牛囊胚一起培养，所述IVF使用性别分选的精液或常规未分选的精液，然后基于活检进行胚胎性别鉴定。在实验3中，子宫内膜外植体单独培养，或在牛囊胚（n=25）或小鼠囊胚（n=25）的存在下培养。培养后，将外植体快速冷冻并储存在-80 ° C直至RNA提取、qPCR或RNA-Seq。培养牛囊胚增加子宫内膜表达的40个成绩单，所有这些都是干扰素tau诱导。与男性或女性牛囊胚培养增加了外植体中5个经典干扰素刺激基因（MX 1，MX2，ISG 15，OASY 1，RSAD 2）的转录丰度;然而，先前报道的与胚胎性别相关的转录丰度（IFNAR 1，IFNAR 2，CTGF，ARTN，SLC 2A 1，SLC 2A 5）没有差异。暴露于小鼠囊胚没有引起任何可检测的变化，转录丰度。这些发现，加上我们以前的数据，表明非常本地，干扰素-tau诱导，子宫内膜基因表达的变化发生在胚泡的反应，这种变化是否在随后的妊娠识别中发挥任何作用仍有待确定。
The aims of this study were (i) to investigate changes in the global transcriptome of bovine endometrial explants induced by exposure to blastocysts, (ii) to investigate if male and female blastocysts elicit a differential response in the endometrial transcriptome in vitro, and (iii) to determine whether bovine endometrium responds to the presence of murine embryos. In Experiment 1, endometrial explants from the same uterus were cultured for 6 h with or without 20 in vitro produced bovine blastocysts. In Experiment 2, endometrial explants were cultured with male or female bovine blastocysts produced in vitro by IVF either using sex-sorted semen or conventional unsorted semen followed by embryo sexing based on a biopsy. In Experiment 3, endometrial explants were cultured alone, or in the presence of bovine blastocysts (n=25) or murine blastocysts (n=25). Following culture, explants were snap frozen and stored at -80°C until RNA extraction, qPCR or RNA-Seq. Culture with bovine blastocysts increased endometrial expression of 40 transcripts, all of which were interferon tau-induced. Culture with male or female bovine blastocysts increased transcript abundance of five classic interferon-stimulated genes (MX1, MX2, ISG15, OASY1, RSAD2) in explants; however, there was no difference in abundance of transcripts previously reported to be related to embryonic sex (IFNAR1, IFNAR2, CTGF, ARTN, SLC2A1, SLC2A5). Exposure to murine blastocysts did not elicit any detectable change in transcript abundance. These findings, coupled with our previous data, indicate that very local, interferon-tau-induced, changes in endometrial gene expression occur in response to blastocysts; whether such changes play any role in subsequent pregnancy recognition remains to be established.
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影响因子：
--
作者：
Su, Ren-Wei;Fazleabas, Asgerally T.
通讯作者：
Fazleabas, Asgerally T.
影响因子：
3.6
作者：
Mathew, Daniel J.;Sanchez, Jose M.;Lonergan, Patrick
通讯作者：
Lonergan, Patrick
DOI：
10.1073/pnas.0811841106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Bauersachs, Stefan;Ulbrich, Susanne E.;Wolf, Eckhard
通讯作者：
Wolf, Eckhard
体内和体外牛囊胚分泌干扰素 tau 的遗传和环境决定因素。
影响因子：
2.2
作者：
Kubisch,HM;Larson,MA;Ealy,AD;Murphy,CN;Roberts,RM
通讯作者：
Roberts,RM