Pod1 is involved in the sexual differentiation and gonadal development of the Nile tilapia
Pod1 is involved in the sexual differentiation and gonadal development of the Nile tilapia
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Pod1 参与尼罗罗非鱼的性别分化和性腺发育
DOI：
10.1007/s11427-011-4240-4
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Nagahama, Yoshitaka
中科院分区：
文献类型：
--
作者：
Li JianZhong;Liu ShaoJun;Nagahama, Yoshitaka
Pod1是碱性螺旋-环-螺旋(BHLH)转录因子家族的成员，参与调节哺乳动物的性分化和性腺发育。然而，到目前为止，关于Pod1在非哺乳动物脊椎动物性腺发生中的作用还知之甚少。我们从罗非鱼中克隆并鉴定了Pod1基因。罗非鱼Pod1基因含有525个核苷酸的开放阅读框(ORF)，编码174个氨基酸的蛋白质。序列比对表明，所推导的罗非鱼蛋白序列与其他脊椎动物的Pod1序列具有较高的同源性(79.5%~90.5%)。RT-PCR显示Pod1在成体罗非鱼中的组织分布具有不同的表达模式。利用原位杂交技术检测Pod1在罗非鱼性分化和性腺发育过程中的时空表达模式。在未分化的性腺中，Pod1在两性的体细胞中均有表达。随后，Pod1在罗非鱼中的表达持续存在于分化的幼鱼和成鱼卵巢和睾丸中。我们的数据首次表明，Pod1不仅是硬骨鱼性别分化的开始所必需的，而且在硬骨鱼的性腺发育中也起着重要的作用。
Pod1 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that have been implicated in the regulation of sexual differentiation and gonadal development in mammals. However, to date, little is known about the role of Pod1 in nonmammalian vertebrate gonadogenesis. We cloned and characterized thePod1gene from tilapia. The tilapiaPod1gene contains an open reading frame (ORF) of 525 nucleotides which potentially codes for a protein with 174 amino acids. Sequence alignment revealed that the deduced tilapia protein sequence shared high homology (79.5% to 90.5%) with the Pod1 sequences of other vertebrates. The tissue distribution ofPod1revealed by RT-PCR showed that it had varied expression patterns in adult tilapia.In situhybridization was performed to examine the temporal and spatial expression patterns of Pod1 during tilapia sexual differentiation and gonadal development. In the undifferentiated gonad,Pod1was expressed in the somatic cells of both sexes. Subsequently, Pod1 expression in tilapia persisted in differentiated juvenile and adult ovary and testis. Our data indicate for the first time that Pod1 is not only necessary for the onset of sexual differentiation, but also plays an important role in gonadal development in the teleost.
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影响因子：
2.5
作者：
Kobayashi, Tohru;Kajiura-Kobayashi, Hiroko;Nagahama, Yoshitaka
通讯作者：
Nagahama, Yoshitaka
DOI：
10.1387/ijdb.051982ss
发表时间：
2005
期刊：
The International journal of developmental biology
影响因子：
--
作者：
S. Simrick;K. Massé;E. Jones
通讯作者：
S. Simrick;K. Massé;E. Jones
影响因子：
2.5
作者：
Cui, SY;Schwartz, L;Quaggin, SE
通讯作者：
Quaggin, SE
影响因子：
2.6
作者：
Quaggin, SE;Vanden Heuvel, GB;Igarashi, P
通讯作者：
Igarashi, P
影响因子：
4.6
作者：
Cui, SY;Ross, A;Quaggin, SE
通讯作者：
Quaggin, SE