Identification and expansion of a unique stem cell population from adult mouse gallbladder.
Identification and expansion of a unique stem cell population from adult mouse gallbladder.
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DOI：
10.1002/hep.24568
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发表时间：
2011-11
期刊：
影响因子：
13.5
通讯作者：
Lagasse, Eric
中科院分区：
文献类型：
--
作者：
Manohar, Rohan;Komori, Junji;Guzik, Lynda;Stolz, Donna B.;Chandran, Uma R.;LaFramboise, William A.;Lagasse, Eric
到目前为止，在小鼠胆囊中鉴定常驻干细胞尚未探索。此外，成人胆囊干细胞和肝内胆管（IHBD）细胞之间的关系还不清楚。本研究的目的是从成年小鼠胆囊中分离干细胞，并确定它们与IHBD细胞相比是否独特。通过有限稀释分析和指数分类，我们发现与EpCAM+ CD 49 flo细胞相比，来自原发性胆囊的EpCAM + CD 49 fhi亚群富集集落形成细胞。EpCAM+ CD 49 fhi细胞表达CD 29、CD 133和Sca 1，但对谱系标志物CD 31、CD 45和F4/80呈阴性。使用新的饲养细胞培养系统，我们观察到体外EpCAM+ CD 49 fhi细胞的长期（> 20代）和克隆扩增。在基质胶分化测定中，体外扩增的EpCAM+ CD 49 f+细胞经历器官型形态发生，形成小管结构和囊肿。这些结构与原发性胆囊的运输功能相似，并概括了原发性胆囊的运输功能。EpCAM+ CD 49 f+细胞也植入受体小鼠的皮下空间。我们通过流式细胞术比较了原代胆囊和IHBD细胞，发现CD 49 f、CD 49 e、CD 81、CD 26、CD 54和CD 166表达的表型差异。此外，寡核苷酸微阵列显示扩增的EpCAM+ CD 49 f+胆囊细胞和IHBD细胞表现出与脂质和药物代谢相关的差异。值得注意的基因是不同的细胞色素P450，谷胱甘肽-S-转移酶，印度刺猬和溶质载体家族基因。我们已经从具有干细胞特征的原代小鼠胆囊中分离出上皮细胞群，并发现其与IHBD细胞相比是独特的。
The identification of resident stem cells in the mouse gallbladder is to date, unexplored. In addition, the relationship between adult gallbladder stem cells and intrahepatic bile duct (IHBD) cells is not well understood. The goal of this study was to isolate stem cells from an adult mouse gallbladder and determine if they were unique compared to IHBD cells. By limiting dilution analyses and index sorts, we found that an EpCAM+CD49fhi sub-population from primary gallbladder is enriched in colony forming cells compared to EpCAM+CD49flo cells. EpCAM+CD49fhi cells expressed CD29, CD133 and Sca1 but were negative for lineage markers CD31, CD45 and F4/80. Using a novel feeder cell culture system, we observed long-term (>passage 20) and clonal expansion of the EpCAM+CD49fhi cells in vitro. In a matrigel differentiation assay, EpCAM+CD49f+ cells expanding in vitro underwent organotypic morphogenesis forming ductular structures and cysts. These structures are similar to, and recapitulate a transport function of primary gallbladder. EpCAM+CD49f+ cells also engraft into the subcutaneous space of recipient mice. We compared primary gallbladder and IHBD cells by flow cytometry and found phenotypic differences in expression of CD49f, CD49e, CD81, CD26, CD54 and CD166. In addition, oligonucleotide microarrays showed that the expanded EpCAM+CD49f+ gallbladder cells and IHBD cells exhibit differences related to lipid and drug metabolism. Notable genes that were different are cytochrome P450, glutathione-S-transferase, Indian hedgehog and solute carrier family genes. we have isolated an epithelial cell population from primary mouse gallbladder with stem cell characteristics and found it to be unique compared to IHBD cells.
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DOI：
10.1083/jcb.98.3.1026
发表时间：
1984-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ehmann UK;Peterson WD Jr;Misfeldt DS
通讯作者：
Misfeldt DS
DOI：
10.1073/pnas.89.7.2804
发表时间：
1992-04-01
影响因子：
11.1
作者：
BAUM, CM;WEISSMAN, IL;PEAULT, B
通讯作者：
PEAULT, B
影响因子：
13.5
作者：
Baumann, U;Crosby, HA;Strain, AJ
通讯作者：
Strain, AJ
影响因子：
11.8
作者：
Spence, Jason R.;Lange, Alex W.;Lin, Suh-Chin J.;Kaestner, Klaus H.;Lowy, Andrew M.;Kim, Injune;Whitsett, Jeffrey A.;Wells, James M.
通讯作者：
Wells, James M.
影响因子：
10.1
作者：
Christ, Oliver;Lucke, Kai;Eaves, Connie
通讯作者：
Eaves, Connie