Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee.
Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee.
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DOI：
10.4062/biomolther.2014.020
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发表时间：
2014-05
影响因子：
3.7
通讯作者：
Hwang SC
中科院分区：
文献类型：
--
作者：
Kang BJ;Ryu J;Lee CJ;Hwang SC
我们研究了毛地黄黄酮是否影响原代培养的兔关节软骨细胞中基质金属蛋白酶-3（MMP-3）的基因表达、分泌和活性，以及大鼠膝关节中MMP-3的产生，以评估毛地黄黄酮潜在的软骨保护作用。体外培养兔关节软骨细胞，采用逆转录-聚合酶链反应（RT-PCR）检测IL-1β诱导的MMP-3、MMP-1、MMP-13、血小板反应蛋白基序的去整合素和金属蛋白酶-4（ADAMTS-4）、ADAMTS-5和II型胶原基因表达水平。采用蛋白质印迹法和酪蛋白酶谱法研究木犀草素对白细胞介素1β（IL 1β）诱导的兔关节软骨细胞MMP 3分泌和酶活性的影响。还在体内检查了木犀草素对MMP-3蛋白产生的影响。结果表明：（1）毛地黄黄酮可抑制MMP-3、MMP-1、MMP-13、ADAMTS-4和ADAMTS-5基因表达水平。但能增加兔关节软骨细胞胶原基因表达水平;（2）木犀草素抑制MMP-3的分泌和活性;（3）木犀草素抑制体内MMP-3蛋白的产生。结果提示，毛地黄黄酮可直接作用于关节软骨细胞，调节MMP-3的基因表达、分泌和活性。
We investigated whether luteolin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as production of MMP-3 in the rat knee to evaluate the potential chondro-protective effects of luteolin. Rabbit articular chondrocytes were cultured in a monolayer and IL-1β-induced gene expression levels of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen were measured by reverse transcription - polymerase chain reaction (RT-PCR). Effects of luteolin on interleukin-1β (IL-1β)-induced secretion and enzyme activity of MMP-3 in rabbit articular chondrocytes were investigated by western blot analysis and casein zymography, respectively. The effect of luteolin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) luteolin inhibited the gene expression levels of MMP-3, MMP-1, MMP-13, ADAMTS-4 and ADAMTS-5. However, it increased the gene expression level of collagen in rabbit articular chondrocytes; (2) luteolin inhibited the secretion and activity of MMP-3; (3) luteolin inhibited in vivo production of MMP-3 protein. These results suggest that luteolin can regulate the gene expression, secretion and activity of MMP-3, by directly acting on articular chondrocytes.
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影响因子：
3.9
作者：
Lee, Je-Hyuk;Kim, Gun-Hee
通讯作者：
Kim, Gun-Hee
影响因子：
27.4
作者：
Goldring MB;Otero M;Tsuchimochi K;Ijiri K;Li Y
通讯作者：
Li Y
影响因子：
27.4
作者：
Freemont, AJ;Hampson, V;Hoyland, JA
通讯作者：
Hoyland, JA
影响因子：
4.3
作者：
Jung, Hyun Ah;Jin, Seong Eun;Choi, Jae Sue
通讯作者：
Choi, Jae Sue
影响因子：
15.9
作者：
DEAN, DD;MARTELPELLETIER, J;WOESSNER, JF
通讯作者：
WOESSNER, JF