Morphological Characterization and Integrated Transcriptome and Proteome Analysis of Organ Development Defective 1 (odd1) Mutant in Cucumis sativus L.
Morphological Characterization and Integrated Transcriptome and Proteome Analysis of Organ Development Defective 1 (odd1) Mutant in Cucumis sativus L.
复制标题
黄瓜器官发育缺陷1（odd1）突变体的形态特征及转录组和蛋白质组综合分析。
DOI：
10.3390/ijms23105843
复制
发表时间：
2022-05-23
影响因子：
5.6
通讯作者：
Cao, Chenxing
中科院分区：
文献类型：
--
作者：
Han, Jing;Ma, Zengguang;Chen, Linjie;Wang, Zaizhan;Wang, Can;Wang, Lina;Chen, Chunhua;Ren, Zhonghai;Cao, Chenxing
黄瓜葫芦科植物是一种重要的经济作物，具有独特的生长习性和典型的蔓生枝结构。阐明黄瓜生长发育的调控机制对提高黄瓜品质和产量具有重要意义。在这里，我们分离到一个自发的黄瓜突变体器官发育缺陷1（odd 1）的多个形态变化，包括根，株高，茎，叶，雄花和雌花，以及水果。解剖学和细胞学分析表明，与WT相比，odd1的细胞大小和数量都减少，茎尖分生组织（SAM）较小。花粉活力测定、萌发试验和杂交试验表明，odd 1为雌性不育，可能是由于没有胚珠所致。遗传分析表明，odd 1为隐性单基因突变体。使用MutMap策略，发现odd1基因位于5号染色体上。转录组和蛋白质组的综合分析表明，与激素和SAM维持相关的基因的差异表达可能是odd 1表型变化的原因。这些结果为黄瓜器官生长发育的分子调控研究提供了新的思路，也为黄瓜的进一步研究提供了参考图谱。
Cucumber (Cucumis sativus L.) is an economically important vegetable crop with the unique growth habit and typical trailing shoot architecture of Cucurbitaceae. Elucidating the regulatory mechanisms of growth and development is significant for improving quality and productivity in cucumber. Here we isolated a spontaneous cucumber mutant organ development defective 1 (odd1) with multiple morphological changes including root, plant stature, stem, leaf, male and female flowers, as well as fruit. Anatomical and cytological analyses demonstrated that both cell size and number decreased, and the shoot apical meristem (SAM) was smaller in odd1 compared with WT. Pollen vigor and germination assays and cross tests revealed that odd1 is female sterile, which may be caused by the absence of ovules. Genetic analysis showed that odd1 is a recessive single gene mutant. Using the MutMap strategy, the odd1 gene was found to be located on chromosome 5. Integrated profiling of transcriptome and proteome indicated that the different expression genes related to hormones and SAM maintenance might be the reason for the phenotypic changes of odd1. These results expanded the insight into the molecular regulation of organ growth and development and provided a comprehensive reference map for further studies in cucumber.
登录
查看更多内容
影响因子：
3.5
作者：
Gou C;Zhu P;Meng Y;Yang F;Xu Y;Xia P;Chen J;Li J
通讯作者：
Li J
影响因子：
4.6
作者：
Dinneny, JR;Yadegari, R;Weigel, D
通讯作者：
Weigel, D
影响因子：
4.6
作者：
Dinneny, JR;Weigel, D;Yanofsky, MF
通讯作者：
Yanofsky, MF
精细定位将编码组蛋白乙酰转移酶的 CsGCN5 确定为黄瓜中 tentril-less1 突变 (td-1) 的假定候选基因
影响因子：
5.4
作者：
Chen, Feifan;Fu, Bingbing;Li, Yuhong
通讯作者：
Li, Yuhong
影响因子：
4.6
作者：
Haecker, A;Gross-Hardt, R;Laux, T
通讯作者：
Laux, T