Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.
Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.
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DOI：
10.1902/jop.2009.090185
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发表时间：
2009-09
影响因子：
4.3
通讯作者：
Paster BJ
中科院分区：
文献类型：
--
作者：
Colombo AP;Boches SK;Cotton SL;Goodson JM;Kent R;Haffajee AD;Socransky SS;Hasturk H;Van Dyke TE;Dewhirst F;Paster BJ
本研究使用人类口腔微生物识别微阵列 (HOMIM) 将难治性牙周炎 (RP) 受试者的龈下微生物群与可治疗牙周炎 (GR) 或牙周健康 (PH) 受试者的龈下微生物群进行了比较。在基线时，从 47 名牙周炎患者和 20 名 PH 个体中采集龈下菌斑样本，并通过 HOMIM 分析是否存在 300 种菌斑。牙周炎受试者根据平均附着丧失（AL）和/或在 SRP、手术和全身施用阿莫西林和甲硝唑后 3 个部位 AL ≥2.5 mm 被分类为 RP（n=17），或根据平均附着增益且治疗后没有 AL ≥2.5 mm 部位被分类为 GR（n=30）。使用 Kruskal Wallis 和卡方检验来寻找群体之间分类单元的显着差异。在患病患者（GR 或 RP）中检测到的物种比未患病患者（PH）中检测到的物种更多。 RP 受试者与 GR 和 PH 的区别在于，假定的牙周病原体频率显着较高，例如微小细单胞菌、纤细弯曲杆菌、结节真杆菌、月光单胞菌、连翘坦氏菌、牙龈卟啉单胞菌、普雷沃菌属、密螺旋体属、腐蚀艾肯氏菌以及“不寻常”物种 （溶乳假杆菌、TM7 spp.口腔分类群 (OT) 346/356、拟杆菌属 OT 272/274、莫雷梭菌、Desulfobulbus sp. OT 041、Brevundimonas diminuta、Sphaerocytophaga sp. OT 337、Shuttleworthia satelles、 Filifactor alocis、Dialister invisus/pneumosintes、Granulicatella adiacens、Mogibacter tidmidum、Veillonella atypica、唾液支原体、Synergistes sp。簇 II，氨基酸球菌科 [G-1] sp。 OT 132/150/155/148/135) [p<0.05]。 PH 患者中比牙周炎患者中更常见的物种包括放线菌属 (Actinomyces sp.)。 OT 170，放线菌属。簇 I、痰二氧化碳嗜纤维菌、人心杆菌、副流感嗜血杆菌、奇异果、丙酸丙酸杆菌、牙齿罗氏菌/粘液菌、血链球菌 (p<0.05)。根据 HOMIM 测定，与 GR 和 PH 组患者相比，RP 患者呈现出独特的微生物特征。
This study compared the subgingival microbiota of subjects with refractory periodontitis (RP) to those in subjects with treatable periodontitis (GR) or periodontal health (PH) using the Human Oral Microbe Identification Microarray (HOMIM). At baseline, subgingival plaque samples were taken from 47 periodontitis and 20 PH individuals, and analyzed for the presence of 300 species by HOMIM. The periodontitis subjects were classified as RP (n=17) based on mean attachment loss (AL) and/or >3 sites with AL ≥2.5 mm after SRP, surgery and systemically administered amoxicillin and metronidazole or as GR (n=30) based on mean attachment gain and no sites with AL ≥2.5 mm after treatment. Significant differences in taxa among groups were sought using the Kruskal Wallis and Chi-square tests. More species were detected in diseased patients (GR or RP) than those without disease (PH). RP subjects were distinguished from GR and PH by a significantly high frequency of putative periodontal pathogens such as, Parvimonas micra, Campylobacter gracilis, Eubacterium nodatum, Selenomonas noxia, Tannerella forsythia, Porphyromonas gingivalis, Prevotella spp., Treponema spp., Eikenella corrodens, as well as “unusual” species (Pseudoramibacter alactolyticus, TM7 spp. oral taxon (OT) 346/356, Bacteroidetes spp. OT 272/274, Solobacterium moorei, Desulfobulbus sp. OT 041, Brevundimonas diminuta, Sphaerocytophaga sp. OT 337, Shuttleworthia satelles, Filifactor alocis, Dialister invisus/pneumosintes, Granulicatella adiacens, Mogibacterium tidmidum, Veillonella atypica, Mycoplasma salivarium, Synergistes sp. cluster II, Acidaminococcaceae [G-1] sp. OT 132/150/155/148/135) [p<0.05]. Species that were more prevalent in PH than in periodontitis patients included Actinomyces sp. OT 170, Actinomyces spp. cluster I, Capnocytophaga sputigena, Cardiobacterium hominis, Haemophilus parainfluenzae, Lautropia mirabilis, Propionibacterium propionicum, Rothia dentocariosa/mucilagenosa, Streptococcus sanguinis (p<0.05). RP patients present a distinct microbial profile compared to patients in the GR and PH groups as determined by HOMIM.
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影响因子：
18.6
作者：
Haffajee, Anne D.;Teles, Ricardo P.;Socransky, Sigmund S.
通讯作者：
Socransky, Sigmund S.
影响因子：
3.1
作者：
CHOI, BK;PASTER, BJ;GOBEL, UB
通讯作者：
GOBEL, UB
影响因子：
4.3
作者：
COLLINS, JG;OFFENBACHER, S;ARNOLD, RR
通讯作者：
ARNOLD, RR
影响因子：
6.7
作者：
Colombo, AP;Haffajee, AD;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
2.8
作者：
GERNERSMIDT, P;KEISERNIELSEN, H;YING, YT
通讯作者：
YING, YT