UV activation of polymeric high aspect ratio microstructures: ramifications in antibody surface loading for circulating tumor cell selection.
UV activation of polymeric high aspect ratio microstructures: ramifications in antibody surface loading for circulating tumor cell selection.
复制标题
聚合物高宽高比微观结构的紫外线激活：抗体表面载荷中的分析，用于循环肿瘤细胞选择。
DOI：
10.1039/c3lc50618e
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发表时间：
2014-01-07
期刊：
影响因子：
6.1
通讯作者：
Soper SA
中科院分区：
文献类型：
--
作者：
Jackson JM;Witek MA;Hupert ML;Brady C;Pullagurla S;Kamande J;Aufforth RD;Tignanelli CJ;Torphy RJ;Yeh JJ;Soper SA
使用稳健且有效的方法活化热塑性表面的需要已经由以下事实驱动：复制技术可以用于以高生产模式和低成本生产微流体装置，使得聚合物微流体对于体外诊断（例如循环肿瘤细胞（CTC）分析）是无价的，其中装置可处置性对于减轻与样品残留相关的伪影是至关重要的。通过活化技术修饰热塑性装置的表面化学可用于增加表面的润湿性或产生功能性支架，以允许生物制剂（例如用于CTC识别的抗体）的共价连接。广泛的表面表征工具被用来研究各种表面的UV活化，以产生均匀和高的表面覆盖度的官能团，如不同纵横比的微通道中的羧酸。我们发现，UV活化过程的效率高度依赖于微通道的纵横比和热塑性基板的身份。在Cy 3标记的寡核苷酸的EDC/NHS偶联之后，UV活化的微通道的比色测定和荧光成像表明，与上部相比，具有10.3的纵横比的PMMA微通道朝向通道底部的UV活化效率显著较低。这种效应是本体聚合物由于吸收伪影而阻尼改性UV辐射的结果。相比之下，COC的这种效果不太明显。此外，我们观察到，在将器件的盖板热熔融结合到衬底之后，许多生成的官能团被埋在本体中，使得它们不可接近。这种表面重组的倾向被认为是更高的PMMA相比，COC。作为材料和微通道纵横比对装置功能性的影响的实例，评估了用于从全血中选择CTC的热塑性装置，其需要将单克隆抗体固定到通道壁。根据我们的结果，我们得出结论，分离的CTC的CTC产率和纯度取决于基质材料，与PMMA相比，COC产生CTC的最高临床产率以及更好的纯度。
The need to activate thermoplastic surfaces using robust and efficient methods has been driven by the fact that replication techniques can be used to produce microfluidic devices in a high production mode and at low cost, making polymer microfluidics invaluable for in vitro diagnostics, such as circulating tumor cell (CTC) analysis, where device disposability is critical to mitigate artifacts associated with sample carryover. Modifying the surface chemistry of thermoplastic devices through activation techniques can be used to increase the wettability of the surface or to produce functional scaffolds to allow for the covalent attachment of biologics, such as antibodies for CTC recognition. Extensive surface characterization tools were used to investigate UV activation of various surfaces to produce uniform and high surface coverage of functional groups, such as carboxylic acids in microchannels of different aspect ratios. We found that the efficiency of the UV activation process is highly dependent on the microchannel aspect ratio and the identity of the thermoplastic substrate. Colorimetric assays and fluorescence imaging of UV-activated microchannels following EDC/NHS coupling of Cy3-labeled oligonucleotides indicated that UV-activation of a PMMA microchannel with an aspect ratio of ∼3 was significantly less efficient toward the bottom of the channel compared to the upper sections. This effect was a consequence of the bulk polymer's damping of the modifying UV radiation due to absorption artifacts. In contrast, this effect was less pronounced for COC. Moreover, we observed that after thermal fusion bonding of the device's cover plate to the substrate, many of the generated functional groups buried into the bulk rendering them inaccessible. The propensity of this surface reorganization was found to be higher for PMMA compared to COC. As an example of the effects of material and microchannel aspect ratios on device functionality, thermoplastic devices for the selection of CTCs from whole blood were evaluated, which required the immobilization of monoclonal antibodies to channel walls. From our results, we concluded the CTC yield and purity of isolated CTCs were dependent on the substrate material with COC producing the highest clinical yields for CTCs as well as better purities compared to PMMA.
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影响因子：
7.4
作者：
Bhattacharyya, A;Klapperich, CM
通讯作者：
Klapperich, CM
影响因子：
2.8
作者：
Hupert, Mateusz L.;Guy, W. Jason;Soper, Steven A.
通讯作者：
Soper, Steven A.
DOI：
10.1007/s00170-009-2345-8
发表时间：
2010-06-01
影响因子：
3.4
作者：
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通讯作者：
Alcock, Jeffrey R.
影响因子：
11.2
作者：
Eliane, Jean-Pierre;Repollet, Madeline;Luker, Gary D.
通讯作者：
Luker, Gary D.
影响因子：
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作者：
Do, Jaephil;Lee, Sehwan;Ahn, Chong H.
通讯作者：
Ahn, Chong H.