Histological assessment of cortical bone changes in diabetic rats.
Histological assessment of cortical bone changes in diabetic rats.
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DOI：
10.1186/s13018-022-03471-0
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发表时间：
2022-12-27
影响因子：
2.6
通讯作者：
Takahashi, Kenji
中科院分区：
文献类型：
--
作者：
Minami, Masataka;Ikoma, Kazuya;Onishi, Okihiro;Horii, Motoyuki;Itoh, Kyoko;Takahashi, Kenji
糖尿病可导致骨强度下降，其组织学机制尚不清楚。我们假设皮质骨的组织学评估将使我们能够确定与糖尿病相关的骨强度降低的原因。我们的目的是评估与糖尿病患者内在骨特性和骨质量恶化相关的皮质骨组织形态计量学变化。我们比较了40周龄糖尿病雄性大鼠和对照雄性大鼠胫骨的机械测试、微计算机断层扫描测量的骨密度和组织学评估的结果，这些结果是通过应用Villanueva骨染色进行的。在力学测试方面，糖尿病患者的最大载荷和能量吸收显著低于对照组，尽管两组之间的骨折位移和刚度没有显著差异。糖尿病组的骨密度显著高于对照组。骨组织形态计量学显示，与对照组相比，糖尿病大鼠骨细胞较少，皮质骨孔隙率较高，皮质骨矿化增加。在糖尿病患者中，具有更大皮质孔隙率的皮质骨矿化增加导致骨强度减弱。
Diabetes mellitus weakens bone strength due to deterioration of bone quality; however, the histological mechanisms are still unknown. We hypothesized that histological assessment of cortical bone would enable us to determine the cause of the bone strength reduction associated with diabetes mellitus. Our aim was to evaluate the histomorphometric changes of cortical bone associated with deterioration of intrinsic bone properties and bone quality in diabetes mellitus. We compared the outcomes of mechanical tests, bone mineral density measured using micro-computed tomography, and histological assessments, by applying Villanueva’s bone stain, to the tibial bones of 40-week-old diabetic and control male rats. With respect to mechanical testing, the maximum load and energy absorption were significantly lower in the diabetic than in the control group, although fracture displacement and stiffness were not significantly different between the two groups. Bone mineral density was significantly higher in the diabetic group than in the control group. Bone histomorphometry revealed that the diabetic rats had fewer osteocytes, greater cortical porosity, and increased mineralization in cortical bone compared with the control group. Increased mineralization of the cortical bone with greater cortical porosity leads to a weakening of bone strength in diabetes mellitus.
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影响因子：
13.6
作者：
Ma, Lili;Oei, Ling;Jiang, Lindi;Estrada, Karol;Chen, Huiyong;Wang, Zhen;Yu, Qiang;Zillikens, Maria Carola;Gao, Xin;Rivadeneira, Fernando
通讯作者：
Rivadeneira, Fernando
影响因子：
5.1
作者：
Ogurtsova, K.;Fernandes, J. D. da Rocha;Makaroff, L. E.
通讯作者：
Makaroff, L. E.
影响因子：
4.3
作者：
Saito, Mitsuru;Kida, Yoshikuni;Marumo, Keishi
通讯作者：
Marumo, Keishi
影响因子：
4.4
作者：
Minami, Masataka;Ikoma, Kazuya;Kubo, Toshikazu
通讯作者：
Kubo, Toshikazu
影响因子：
2.9
作者：
Saito, M;Marumo, K;Ishioka, N
通讯作者：
Ishioka, N