The two Cis-acting sites, parS1 and oriC1, contribute to the longitudinal organisation of Vibrio cholerae chromosome I.
The two Cis-acting sites, parS1 and oriC1, contribute to the longitudinal organisation of Vibrio cholerae chromosome I.
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DOI：
10.1371/journal.pgen.1004448
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发表时间：
2014-07
期刊：
影响因子：
4.5
通讯作者：
Possoz C
中科院分区：
文献类型：
--
作者：
David A;Demarre G;Muresan L;Paly E;Barre FX;Possoz C
细菌染色体的分离遵循空间组织的精确编排。它是由复制起点（ori）的姐妹拷贝的双极迁移启动的。大多数细菌染色体包含一个分区系统（Par）与parS网站在接近ori，有助于积极动员的ori区域向旧极。这被认为导致细胞内的染色体纵向排列。在这项研究中，我们遵循的复制频率和细胞位置的19个霍乱弧菌基因组位点作为细胞长度的函数。霍乱弧菌的基因组分为两条染色体，染色体I和II，两者都含有Par系统。染色体I的ori区域（oriI）被束缚在旧极，而染色体II的ori区域被发现在中细胞。然而，我们发现，这两个染色体采用纵向组织。染色体I延伸到整个细胞，而染色体II延伸到年轻的一半细胞。我们进一步证明，位移parS网站远离oriI区域旋转染色体I的大部分。唯一的例外是复制终止的区域，它仍然局限于隔膜。然而，纵向排列的染色体I坚持在Par突变体，如前所述，ori区域仍然局限于对旧极。最后，我们表明，独立于PAR的纵向组织和OriI极性扰动的第二个起源的引入。两者合计，这些结果表明，Par系统是主要贡献者的纵向组织的染色体I，但复制程序也影响细菌染色体的排列。
The segregation of bacterial chromosomes follows a precise choreography of spatial organisation. It is initiated by the bipolar migration of the sister copies of the replication origin (ori). Most bacterial chromosomes contain a partition system (Par) with parS sites in close proximity to ori that contribute to the active mobilisation of the ori region towards the old pole. This is thought to result in a longitudinal chromosomal arrangement within the cell. In this study, we followed the duplication frequency and the cellular position of 19 Vibrio cholerae genome loci as a function of cell length. The genome of V. cholerae is divided between two chromosomes, chromosome I and II, which both contain a Par system. The ori region of chromosome I (oriI) is tethered to the old pole, whereas the ori region of chromosome II is found at midcell. Nevertheless, we found that both chromosomes adopted a longitudinal organisation. Chromosome I extended over the entire cell while chromosome II extended over the younger cell half. We further demonstrate that displacing parS sites away from the oriI region rotates the bulk of chromosome I. The only exception was the region where replication terminates, which still localised to the septum. However, the longitudinal arrangement of chromosome I persisted in Par mutants and, as was reported earlier, the ori region still localised towards the old pole. Finally, we show that the Par-independent longitudinal organisation and oriI polarity were perturbed by the introduction of a second origin. Taken together, these results suggest that the Par system is the major contributor to the longitudinal organisation of chromosome I but that the replication program also influences the arrangement of bacterial chromosomes.
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