Identification and Functional Analysis of ThADH1 and ThADH4 Genes Involved in Tolerance to Waterlogging Stress in Taxodium hybrid 'Zhongshanshan 406'.
Identification and Functional Analysis of ThADH1 and ThADH4 Genes Involved in Tolerance to Waterlogging Stress in Taxodium hybrid 'Zhongshanshan 406'.
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红豆杉杂交种'中山山406'耐涝胁迫相关基因ThADH 1和ThADH 4的鉴定及功能分析
DOI：
10.3390/genes12020225
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发表时间：
2021-02-04
期刊：
影响因子：
3.5
通讯作者：
Creech DL
中科院分区：
文献类型：
--
作者：
Xuan L;Hua J;Zhang F;Wang Z;Pei X;Yang Y;Yin Y;Creech DL
红豆杉杂交种'中山山406'（T.杂交种'中山山406'）[墨西哥红豆杉×二列红豆杉（Taxacumdistichum（L.）[Rich]具有突出的耐淹优势，在我国湿地造林中得到广泛应用。乙醇脱氢酶基因（ADH）在低氧条件下维持植物能量供应的乙醇代谢中起着关键作用。根据T.杂交种‘中山山406’在淹水胁迫下生长。然后通过瞬时表达和过表达的方法研究这两个基因的功能。结果表明，ThADH 1和ThADH 4蛋白均属于ADH III亚家族。ThADH 1定位于细胞质和细胞核，而ThADH 4仅定位于细胞质。这两个基因在根中的表达量显著高于茎和叶。ThADH 1和ThADH 4在杨树中的过量表达导致了相反的表型，但两种转基因杨树的耐湿性显著提高。这些结果表明，ThADH 1和ThADH 4基因参与了T.杂交种‘中山山406’。
The Taxodium hybrid ‘Zhongshanshan 406’ (T. hybrid ‘Zhongshanshan 406’) [Taxodium mucronatum Tenore × Taxodium distichum (L.). Rich] has an outstanding advantage in flooding tolerance and thus has been widely used in wetland afforestation in China. Alcohol dehydrogenase genes (ADHs) played key roles in ethanol metabolism to maintain energy supply for plants in low-oxygen conditions. Two ADH genes were isolated and characterized—ThADH1 and ThADH4 (GenBank ID: AWL83216 and AWL83217—basing on the transcriptome data of T. hybrid ‘Zhongshanshan 406’ grown under waterlogging stress. Then the functions of these two genes were investigated through transient expression and overexpression. The results showed that the ThADH1 and ThADH4 proteins both fall under ADH III subfamily. ThADH1 was localized in the cytoplasm and nucleus, whereas ThADH4 was only localized in the cytoplasm. The expression of the two genes was stimulated by waterlogging and the expression level in roots was significantly higher than those in stems and leaves. The respective overexpression of ThADH1 and ThADH4 in Populus caused the opposite phenotype, while waterlogging tolerance of the two transgenic Populus significantly improved. Collectively, these results indicated that genes ThADH1 and ThADH4 were involved in the tolerance and adaptation to anaerobic conditions in T. hybrid ‘Zhongshanshan 406’.
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